Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus

Nansalmaa Amarsaikhan, Evan M. O'Dea, Angar Tsoggerel, Henry Owegi, Jordan Gillenwater, Steven Templeton

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.

Original languageEnglish
Article numbere100430
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 19 2014

Fingerprint

Chitin
Aspergillus fumigatus
Aspergillus
cell wall components
Pathogens
Cell Wall
Virulence
virulence
Cells
chitin
Glucans
pathogens
Pathology
Growth
Chemical analysis
glucans
Tissue
Germination
morbidity
lungs

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus. / Amarsaikhan, Nansalmaa; O'Dea, Evan M.; Tsoggerel, Angar; Owegi, Henry; Gillenwater, Jordan; Templeton, Steven.

In: PLoS One, Vol. 9, No. 6, e100430, 19.06.2014.

Research output: Contribution to journalArticle

Amarsaikhan, Nansalmaa ; O'Dea, Evan M. ; Tsoggerel, Angar ; Owegi, Henry ; Gillenwater, Jordan ; Templeton, Steven. / Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus. In: PLoS One. 2014 ; Vol. 9, No. 6.
@article{435a351806b74192b6d7d7d0ce4b5a91,
title = "Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus",
abstract = "The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.",
author = "Nansalmaa Amarsaikhan and O'Dea, {Evan M.} and Angar Tsoggerel and Henry Owegi and Jordan Gillenwater and Steven Templeton",
year = "2014",
month = "6",
day = "19",
doi = "10.1371/journal.pone.0100430",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus

AU - Amarsaikhan, Nansalmaa

AU - O'Dea, Evan M.

AU - Tsoggerel, Angar

AU - Owegi, Henry

AU - Gillenwater, Jordan

AU - Templeton, Steven

PY - 2014/6/19

Y1 - 2014/6/19

N2 - The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.

AB - The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype.

UR - http://www.scopus.com/inward/record.url?scp=84903281214&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903281214&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0100430

DO - 10.1371/journal.pone.0100430

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e100430

ER -