Isolation and biochemical characterization of leukemia-associated inhibitory activity that suppresses colony and cluster formation of cells

John Bognacki, Hal E. Broxmeyer, Joseph Lobue

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Leukimia-associated inhibitory activity suppresses colony and cluster formation in vitro of cells derived from granulocyte-macrophage progenitor cells of normal donors. It does not inhibit these same progenitor cells from patients with leukemia and it may contribute to the proliferative advantage leukemia cells appear to possess over normal hematopoietic cells during acute leukemia. The inhibitory activity was isolated by a combination of procedures including: ultracentrifugation, Sephadex G-200, carboxymethylcellulose, SDS-polyacrylamide gel electrophoresis, thin-layer and preparative isoelectric focusing and concanavalin A-Sepharose. Leukemia-associated inhibitory activity was characterized as a glycoprotein. It was inactivated by trypsin, chymotrypsin, pronase and periodate treatment. It bound to and was eluted by α-methylmannose from concanavalin A-Sepharose columns and had an apparent Mr range of 450-550 000 and an isoelectric focus value between pH 4.6 and 4.9. Crude leukemia associated inhibitory activity was temperature sensitive but the more purified preparations were heat stable.

Original languageEnglish (US)
Pages (from-to)176-190
Number of pages15
JournalBBA - General Subjects
Volume672
Issue number2
DOIs
StatePublished - Jan 21 1981
Externally publishedYes

Keywords

  • Clustering
  • Colony formation
  • Inhibition activity
  • Leukemia

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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