Isolation and cardiovascular activity of a second bradykinin-related peptide ([Arg0,Trp5,Leu8] bradykinin) from trout

J. Michael Conlon, Jean Claude Le Mevel, Daniel Conklin, Leroy Weaver, Douglas W. Duff, Kenneth Olson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Previous work has shown that incubation of heat-denatured plasma from the rainbow trout Oncorhynchus mykiss with porcine pancreatic kallikrein generates [Lys0,Trp5,Leu8]bradykinin (trout [Lys0] BK). We have now isolated a second BK-related peptide from kallikrein-treated trout plasma with the primary structure: Arg-Arg-Pro-Pro-Gly-Trp-Ser-Pro-Leu-Arg (trout [Arg0]BK). Bolus injections of both trout [Arg0]BK and [Lys0]BK (>100 pmol/kg) into the dorsal aorta of conscious trout produced multiphasic effects on arterial blood pressure. An initial pressor response of short duration (1-2 min) was followed by a fall in pressure (to below basal values in 11 out of 15 animals) and then by a sustained rise in pressure lasting up to 60 min. The maximum rise in pressure produced by trout [Arg0] BK (10 nmol/kg) was approximately one-fourth of the maximum rise produced by angiotensin II in the same animals. Intracerebroventricular injections of trout [Arg0]BK (500 pmol) into conscious trout had no effect on arterial blood pressure or heart rate. Trout [Arg0]BK did not affect the tension of vascular rings from trout efferent branchial and caeliacomesenteric arteries and anterior cardinal vein. Trout des [Arg9] BK had no effect on cardiovascular parameters, either in vivo or in vitro, indicating that the C-terminal arginine residue of the peptide is important in interaction with the trout kinin receptor(s).

Original languageEnglish (US)
Pages (from-to)531-537
Number of pages7
JournalPeptides
Volume17
Issue number3
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Trout
Blood pressure
Bradykinin
Animals
Tissue Kallikreins
Plasmas
Peptides
Kinins
Kallikreins
Angiotensin II
Arginine
Oncorhynchus mykiss
Pressure
Arterial Pressure
Injections
Blood Vessels
Aorta
Hot Temperature
poly(prolylprolylglycine)15
Veins

Keywords

  • Bradykinin
  • Cardiovascular regulation
  • Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Isolation and cardiovascular activity of a second bradykinin-related peptide ([Arg0,Trp5,Leu8] bradykinin) from trout. / Conlon, J. Michael; Le Mevel, Jean Claude; Conklin, Daniel; Weaver, Leroy; Duff, Douglas W.; Olson, Kenneth.

In: Peptides, Vol. 17, No. 3, 1996, p. 531-537.

Research output: Contribution to journalArticle

Conlon, J. Michael ; Le Mevel, Jean Claude ; Conklin, Daniel ; Weaver, Leroy ; Duff, Douglas W. ; Olson, Kenneth. / Isolation and cardiovascular activity of a second bradykinin-related peptide ([Arg0,Trp5,Leu8] bradykinin) from trout. In: Peptides. 1996 ; Vol. 17, No. 3. pp. 531-537.
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