Isolation and characterization of a folate receptor from human placenta

Asok Antony, C. Utley, K. C. Van Horne, J. F. Kolhouse

Research output: Contribution to journalArticle

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Abstract

While folate binding proteins have been described in serum and a variety of tissues, the function of these proteins is unknown. A particulate folate binding protein from human placenta has been isolated and characterized following solubilization with Triton X-100. The protein was purified 61,000-fold using affinity chromatography on pteroylglutamic acid-Sepharose as the major purification step. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis the purified protein gave a single band with a M[r] = 38,500. Stoichiometry of binding indicated that 1 mol of folate was bound per mol of protein. The protein was a glycoprotein that contained 12% carbohydrate. Antiserum was raised in a rabbit, and on immunodiffusion, gave a single precipitin line with the purified placental folate binding protein. Immunoprecipitation studies using this antiserum indicated that the purified placental folate binding protein shared antigenic determinants with both the large M[r] and small M[r] folate binding proteins from human milk. Immunofluorescent studies with this antiserum and human erythrocytes revealed the presence of an immunologically similar protein on the plasma membrane of these cells suggesting that this protein may function as a folate receptor.

Original languageEnglish (US)
Pages (from-to)9684-9692
Number of pages9
JournalJournal of Biological Chemistry
Volume256
Issue number18
StatePublished - 1981
Externally publishedYes

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Folic Acid
Placenta
Folate Receptor 2
Immune Sera
Carrier Proteins
Proteins
Affinity chromatography
Precipitins
Octoxynol
Cell membranes
Electrophoresis
Immunodiffusion
Sodium Dodecyl Sulfate
Stoichiometry
Sepharose
Human Milk
Purification
Plasma Cells
Epitopes
Affinity Chromatography

ASJC Scopus subject areas

  • Biochemistry

Cite this

Antony, A., Utley, C., Van Horne, K. C., & Kolhouse, J. F. (1981). Isolation and characterization of a folate receptor from human placenta. Journal of Biological Chemistry, 256(18), 9684-9692.

Isolation and characterization of a folate receptor from human placenta. / Antony, Asok; Utley, C.; Van Horne, K. C.; Kolhouse, J. F.

In: Journal of Biological Chemistry, Vol. 256, No. 18, 1981, p. 9684-9692.

Research output: Contribution to journalArticle

Antony, A, Utley, C, Van Horne, KC & Kolhouse, JF 1981, 'Isolation and characterization of a folate receptor from human placenta', Journal of Biological Chemistry, vol. 256, no. 18, pp. 9684-9692.
Antony, Asok ; Utley, C. ; Van Horne, K. C. ; Kolhouse, J. F. / Isolation and characterization of a folate receptor from human placenta. In: Journal of Biological Chemistry. 1981 ; Vol. 256, No. 18. pp. 9684-9692.
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