Isolation and characterization of a mammalian homolog of the Drosophila white gene

James Croop, George E. Tiller, Jonathan A. Fletcher, Marcia L. Lux, Elizabeth Raab, Dianna Goldenson, Dennis Son, Sandra Arciniegas, Rui Lian Wu

Research output: Contribution to journalArticle

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Abstract

The Drosophila melanogaster white gene is a member of the ABC transporter superfamily of ATPase transmembrane proteins and is involved in the cellular uptake of guanine and tryptophan. We have cloned and sequenced human and mouse homologs of white which share 55-58% amino acid similarity with the Drosophila protein. Northern analysis reveals that the mammalian homolog is highly expressed in several tissues, including brain, spleen, lung and placenta. We have localized the gene to human chromosome 21q22.3 by means of fluorescence in situ hybridization and linkage analysis using a (CA)(n) polymorphism. The human homolog maps to the interval between D21S212 and D21S171, a region which includes loci for bipolar affective disorder and a recessive form of deafness. Since tryptophan is a precursor for the neurotransmitter serotonin and neurotoxic metabolites of the kynurenine pathway, we propose that the human homolog of white is a suitable candidate gene for these neurological disorders in humans.

Original languageEnglish (US)
Pages (from-to)77-85
Number of pages9
JournalGene
Volume185
Issue number1
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Drosophila
Tryptophan
Genes
Kynurenine
Drosophila Proteins
ATP-Binding Cassette Transporters
Guanine
Human Chromosomes
Deafness
Nervous System Diseases
Drosophila melanogaster
Fluorescence In Situ Hybridization
Mood Disorders
Bipolar Disorder
Placenta
Neurotransmitter Agents
Adenosine Triphosphatases
Serotonin
Spleen
Amino Acids

Keywords

  • ABC transporters
  • Guanine
  • Kynurenine
  • PFKL
  • Serotonin
  • Tryptophan

ASJC Scopus subject areas

  • Genetics

Cite this

Croop, J., Tiller, G. E., Fletcher, J. A., Lux, M. L., Raab, E., Goldenson, D., ... Wu, R. L. (1997). Isolation and characterization of a mammalian homolog of the Drosophila white gene. Gene, 185(1), 77-85. https://doi.org/10.1016/S0378-1119(96)00633-6

Isolation and characterization of a mammalian homolog of the Drosophila white gene. / Croop, James; Tiller, George E.; Fletcher, Jonathan A.; Lux, Marcia L.; Raab, Elizabeth; Goldenson, Dianna; Son, Dennis; Arciniegas, Sandra; Wu, Rui Lian.

In: Gene, Vol. 185, No. 1, 1997, p. 77-85.

Research output: Contribution to journalArticle

Croop, J, Tiller, GE, Fletcher, JA, Lux, ML, Raab, E, Goldenson, D, Son, D, Arciniegas, S & Wu, RL 1997, 'Isolation and characterization of a mammalian homolog of the Drosophila white gene', Gene, vol. 185, no. 1, pp. 77-85. https://doi.org/10.1016/S0378-1119(96)00633-6
Croop, James ; Tiller, George E. ; Fletcher, Jonathan A. ; Lux, Marcia L. ; Raab, Elizabeth ; Goldenson, Dianna ; Son, Dennis ; Arciniegas, Sandra ; Wu, Rui Lian. / Isolation and characterization of a mammalian homolog of the Drosophila white gene. In: Gene. 1997 ; Vol. 185, No. 1. pp. 77-85.
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