Isolation and characterization of amyloid protein AA in the Abyssinian cat

S. P. DiBartola, Merrill Benson, F. E. Dwulet, J. B. Cornacoff

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Amyloid fibrils were isolated by extraction in deionized water from the kidneys of an Abyssinian cat with familial renal amyloidosis. The fibrils were suspended in a buffer containing 6 M guanidine hydrochloride and reduced and alkylated using dithiothreitol and iodoacetic acid. The resulting amyloid fibril subunit protein was isolated by chromatography on a column of Sepharose CL6B. It was fragmented using cyanogen bromide, and the resultant peptides were separated by reverse phase high performance liquid chromatography. The protein was characterized by determination of the amino acid sequence of the cyanogen bromide fragments using a Beckman 890C sequencer. The primary strucure of this amyloid fibril subunit protein showed strong homology with amyloid protein AA found in man and animals with spontaneous and experimentally induced reactive systemic amyloidosis. This study confirms the reactive nature of familial renal amyloidosis in the Abyssinian cat and suggests that this disease may be a valuable spontaneous animal model for the study of familial Mediterranian fever in man.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalLaboratory Investigation
Volume52
Issue number5
StatePublished - 1985
Externally publishedYes

Fingerprint

Serum Amyloid A Protein
Amyloid
Cyanogen Bromide
Cats
Protein Subunits
Cat Diseases
Iodoacetic Acid
Dithiothreitol
Guanidine
Protein Sequence Analysis
Amyloidosis
Reverse-Phase Chromatography
Sepharose
Chromatography
Buffers
Fever
Animal Models
High Pressure Liquid Chromatography
Kidney
Peptides

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

DiBartola, S. P., Benson, M., Dwulet, F. E., & Cornacoff, J. B. (1985). Isolation and characterization of amyloid protein AA in the Abyssinian cat. Laboratory Investigation, 52(5), 485-489.

Isolation and characterization of amyloid protein AA in the Abyssinian cat. / DiBartola, S. P.; Benson, Merrill; Dwulet, F. E.; Cornacoff, J. B.

In: Laboratory Investigation, Vol. 52, No. 5, 1985, p. 485-489.

Research output: Contribution to journalArticle

DiBartola, SP, Benson, M, Dwulet, FE & Cornacoff, JB 1985, 'Isolation and characterization of amyloid protein AA in the Abyssinian cat', Laboratory Investigation, vol. 52, no. 5, pp. 485-489.
DiBartola, S. P. ; Benson, Merrill ; Dwulet, F. E. ; Cornacoff, J. B. / Isolation and characterization of amyloid protein AA in the Abyssinian cat. In: Laboratory Investigation. 1985 ; Vol. 52, No. 5. pp. 485-489.
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