Isolation and characterization of cDNAs expressed in the early stages of flavonol-induced pollen germination in petunia

Virginie N. Guyon, James D. Astwood, Ethan C. Garner, A. Dunker, Loverine P. Taylor

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Petunia (Petunia hybrida) pollen requires flavonols (Fl) to germinate. Adding kaempferol to Fl-deficient pollen causes rapid and synchronous germination and tube outgrowth. We exploited this system to identify genes responsive to Fls and to examine the changes in gene expression that occur during the first 0.5 h of pollen germination. We used a subtracted library and differential screening to identify 22 petunia germinating pollen clones. All but two were expressed exclusively in pollen and half of the clones were rare or low abundance cDNAs. RNA gel-blot analysis showed that the steady-state transcript levels of all the clones were increased in response to kaempferol. The sequences showing the greatest response to kaempferol encode proteins that have regulatory or signaling functions and include S/D4, a leucine-rich repeat protein, S/D1, a LIM-domain protein, and D14, a putative Zn finger protein with a heme-binding site. Eight of the clones were novel including S/D10, a cDNA only expressed very late in pollen development and highly up-regulated during the first 0.5 h of germination. The translation product of the S/D3 cDNA shares some features with a neuropeptide that regulates guidance and growth in the tips of extending axons. This study confirmed that the bulk of pollen mRNA accumulates well before germination, but that specific sequences are transcribed during the earliest moments of Fl-induced pollen germination.

Original languageEnglish (US)
Pages (from-to)699-710
Number of pages12
JournalPlant Physiology
Volume123
Issue number2
StatePublished - 2000
Externally publishedYes

Fingerprint

Petunia
pollen germination
Germination
Pollen
flavonols
Complementary DNA
pollen
kaempferol
Flavonols
clones
Clone Cells
germination
Petunia hybrida
LIM Domain Proteins
proteins
regulatory proteins
neuropeptides
heme
axons
translation (genetics)

ASJC Scopus subject areas

  • Plant Science

Cite this

Isolation and characterization of cDNAs expressed in the early stages of flavonol-induced pollen germination in petunia. / Guyon, Virginie N.; Astwood, James D.; Garner, Ethan C.; Dunker, A.; Taylor, Loverine P.

In: Plant Physiology, Vol. 123, No. 2, 2000, p. 699-710.

Research output: Contribution to journalArticle

Guyon, Virginie N. ; Astwood, James D. ; Garner, Ethan C. ; Dunker, A. ; Taylor, Loverine P. / Isolation and characterization of cDNAs expressed in the early stages of flavonol-induced pollen germination in petunia. In: Plant Physiology. 2000 ; Vol. 123, No. 2. pp. 699-710.
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