Isolation and characterization of Drosophila multidrug resistance gene homologs

C. Ting Wu, Mark Budding, Mary S. Griffin, James Croop

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Mammalian multidrug-resistant cell lines, selected for resistance to a single cytotoxic agent, display cross-resistance to a broad spectrum of structurally and functionally unrelated compounds. These cell lines overproduce a membrane protein, the P-glycoprotein, which is encoded by a member(s) of a multigene family, termed mdr or pgp. The amino acid sequence of the P-glycoprotein predicts an energy-dependent transport protein with homology to a large superfamily of proteins which transport a wide variety of substances. This report describes the isolation and characterization of two Drosophila homologs of the mammalian mdr gene. These homologs, located in chromosomal sections 49EF and 65A, encode proteins that share over 40% amino acid identity to the human and murine mdr P-glycoproteins. Fly strains bearing disruptions in the homolog in section 49EF have been constructed and implicate this gene in conferring colchicine resistance to the organism. This work sets the foundation for the molecular and genetic analysis of mdr homologs in Drosophila melanogaster.

Original languageEnglish (US)
Pages (from-to)3940-3948
Number of pages9
JournalMolecular and Cellular Biology
Volume11
Issue number8
StatePublished - Aug 1991
Externally publishedYes

Fingerprint

MDR Genes
P-Glycoprotein
Drosophila
P-Glycoproteins
Cell Line
Cytotoxins
Colchicine
Protein Transport
Multigene Family
Drosophila melanogaster
Diptera
Genes
Molecular Biology
Amino Acid Sequence
Carrier Proteins
Membrane Proteins
Amino Acids
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Isolation and characterization of Drosophila multidrug resistance gene homologs. / Wu, C. Ting; Budding, Mark; Griffin, Mary S.; Croop, James.

In: Molecular and Cellular Biology, Vol. 11, No. 8, 08.1991, p. 3940-3948.

Research output: Contribution to journalArticle

Wu, C. Ting ; Budding, Mark ; Griffin, Mary S. ; Croop, James. / Isolation and characterization of Drosophila multidrug resistance gene homologs. In: Molecular and Cellular Biology. 1991 ; Vol. 11, No. 8. pp. 3940-3948.
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