Isolation and characterization of endothelial progenitor cells from human blood

Laura E. Mead, Daniel Prater, Mervin Yoder, David Ingram

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Circulating endothelial progenitor cells (EPCs) in adult human peripheral blood were originally identified in 1997 by Asahara et al., which challenged the paradigm that vasculogenesis is a process restricted to embryonic development. Since their original identification, EPCs have been extensively studied as biomarkers to assess the risk of cardiovascular disease in human subjects and as a potential cell therapeutic for vascular regeneration. Endothelial colony-forming cells (ECFCs), which are a subtype of EPCs, were recently identified from circulating adult and human umbilical cord blood. In contrast to other types of EPCs, which display various monocyte/macrophage phenotypes and functions, ECFCs are characterized by robust proliferative potential, secondary and tertiary colony formation upon replating, and de novo blood vessel formation in vivo when transplanted into immunodeficient mice. In this unit, we describe detailed methodologies for isolation and characterization of ECFCs from both human peripheral and umbilical cord blood. Curr. Protoc. Stem Cell Biol. 6:2C.1.1-2C.1.27. © 2008 by John Wiley & Sons, Inc.

Original languageEnglish
JournalCurrent Protocols in Stem Cell Biology
Issue numberSUPPL. 6
DOIs
StatePublished - 2008

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Fetal Blood
Blood Vessels
Nuclear Family
Embryonic Development
Regeneration
Monocytes
Cardiovascular Diseases
Stem Cells
Biomarkers
Macrophages
Phenotype
Endothelial Progenitor Cells
Therapeutics

Keywords

  • Adult progenitor cells
  • Endothelial cell transplantation
  • Endothelial colony-forming cells (ECFC)
  • Endothelial progenitor cell (EPC)

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Isolation and characterization of endothelial progenitor cells from human blood. / Mead, Laura E.; Prater, Daniel; Yoder, Mervin; Ingram, David.

In: Current Protocols in Stem Cell Biology, No. SUPPL. 6, 2008.

Research output: Contribution to journalArticle

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