Isolation and characterization of LMC, a novel lymphocyte and monocyte chemoattractant human CC chemokine, with myelosuppressive activity

Byung S. Youn, Shangming Zhang, Hal E. Broxmeyer, Kathleen Antol, Malcolm J. Fraser, Giao Hangoc, Byoung S. Kwon

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

By searching the Expressed Sequence Tag (EST) data base, we identified a partial cDNA sequence encoding a novel human CC chemokine. The entire cDNA sequence was determined and revealed a CC chemokine whose mature protein consisted of 100 amino acids with predicted molecular weight of 11 kd. The chemokine preferantially chemoattracted lymphocytes and monocytes but not neutrophils. It was, therefore, named LMC (Lymphocyte and Monocyte Chemoattractant). LMC exhibited potent myelosuppressive activity, which was comparable to that of MIP-1α. We identified several bacterial artificial clones (BAG) containing the LMC gene along with two human CC chemokine subfamily members; leukotactin-1 (Lkn-1) and CKβ8-1/CKβ8. This data suggests that the LMC gene is located at human chromosome 17q which encompasses a human CC chemokine gene cluster.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume247
Issue number2
DOIs
StatePublished - Jun 18 1998

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CC Chemokines
Lymphocytes
Chemotactic Factors
Monocytes
Genes
Complementary DNA
Expressed Sequence Tags
Human Chromosomes
Chromosomes
Multigene Family
Chemokines
Neutrophils
Clone Cells
Molecular Weight
Molecular weight
Databases
Amino Acids
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Isolation and characterization of LMC, a novel lymphocyte and monocyte chemoattractant human CC chemokine, with myelosuppressive activity. / Youn, Byung S.; Zhang, Shangming; Broxmeyer, Hal E.; Antol, Kathleen; Fraser, Malcolm J.; Hangoc, Giao; Kwon, Byoung S.

In: Biochemical and Biophysical Research Communications, Vol. 247, No. 2, 18.06.1998, p. 217-222.

Research output: Contribution to journalArticle

Youn, Byung S. ; Zhang, Shangming ; Broxmeyer, Hal ; Antol, Kathleen ; Fraser, Malcolm J. ; Hangoc, Giao ; Kwon, Byoung S. / Isolation and characterization of LMC, a novel lymphocyte and monocyte chemoattractant human CC chemokine, with myelosuppressive activity. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 247, No. 2. pp. 217-222.
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