Isolation and characterization of murine mandibular condylar cartilage cell populations

J. Chen, Achint Utreja, Z. Kalajzic, T. Sobue, D. Rowe, S. Wadhwa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives: The mandibular condylar cartilage is a heterogeneous tissue containing cells at various stages of chondrocyte maturation organized into 4 zones: superficial, polymorphic, flattened, and hypertrophic. The goal of this study was to use transgenic mice containing chondrocyte maturation markers fused to fluorescent protein transgenes to isolate and characterize homogenous cell populations of the mandibular condylar cartilage. Methods: Fluorescent reporter expression in the mandibular condylar cartilage of transgenic mice containing the 3.6-kb fragment of the rat collagen type 1 promoter fused to a topaz-fluorescent protein (Col3.6-tpz), collagen type 2 promoter fused to a cyan-fluorescent protein (Col2-cyan), and/or collagen type 10 promoter fused to cherry-fluorescent protein (Col10-cherry) was examined. Mandibular condylar cartilage cells were analyzed by fluorescence-activated cell sorting (FACS) and either used for gene expression analysis or plated in cell cultures and exposed to adipogenic, osteogenic, or chondrogenic conditions. To determine cell fate, transgenic mice containing the Col3.6-cre recombinase were bred with cre reporter mice. Results: Localization and analysis of gene expression revealed that Col3.6-tpz-positive cells corresponded to the polymorphic/flattened zones and Col2-cyan-positive cells corresponded to the flattened/hypertrophic zones of the mandibular condylar cartilage. Mandibular condylar cartilage FACS-sorted Col3.6-tpz-positive cells have the potential to differentiate into bone, cartilage, and fat. Cell fate mapping revealed that Col3.6 cells are precursors of some of the hypertrophic chondrocytes in the mandibular condylar cartilage. Conclusion: Col3.6-tpz cells represent an earlier stage of the mandibular condylar cartilage maturation pathway.

Original languageEnglish (US)
Pages (from-to)232-243
Number of pages12
JournalCells Tissues Organs
Volume195
Issue number3
DOIs
StatePublished - Feb 2012
Externally publishedYes

Fingerprint

Cartilage
Population
Chondrocytes
Transgenic Mice
Flow Cytometry
Collagen Type X
Gene Expression
Proteins
Collagen Type II
Collagen Type I
Transgenes
Cell Culture Techniques
Fats
Bone and Bones

Keywords

  • Cell fate
  • Chondrocyte maturation
  • Fibrocartilage
  • Mandibular condylar cartilage
  • Temporomandibular joint

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Chen, J., Utreja, A., Kalajzic, Z., Sobue, T., Rowe, D., & Wadhwa, S. (2012). Isolation and characterization of murine mandibular condylar cartilage cell populations. Cells Tissues Organs, 195(3), 232-243. https://doi.org/10.1159/000325148

Isolation and characterization of murine mandibular condylar cartilage cell populations. / Chen, J.; Utreja, Achint; Kalajzic, Z.; Sobue, T.; Rowe, D.; Wadhwa, S.

In: Cells Tissues Organs, Vol. 195, No. 3, 02.2012, p. 232-243.

Research output: Contribution to journalArticle

Chen, J, Utreja, A, Kalajzic, Z, Sobue, T, Rowe, D & Wadhwa, S 2012, 'Isolation and characterization of murine mandibular condylar cartilage cell populations', Cells Tissues Organs, vol. 195, no. 3, pp. 232-243. https://doi.org/10.1159/000325148
Chen, J. ; Utreja, Achint ; Kalajzic, Z. ; Sobue, T. ; Rowe, D. ; Wadhwa, S. / Isolation and characterization of murine mandibular condylar cartilage cell populations. In: Cells Tissues Organs. 2012 ; Vol. 195, No. 3. pp. 232-243.
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