Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord

Sai Li Fu, Zheng Wen Ma, Lan Yin, Pei Hua Lu, Xiao-Ming Xu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells (NSCs) from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used. The results are as follows. (1) In the presence of fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF), both brain- and spinal cord-derived stem cells proliferated and expanded in vitro for 8-10 passages (over 60 d). The period of expansion resulted in a 10 6-fold increase in brain-derived NSCs and 10 5-fold increase in spinal cord-derived NSCs. These proliferating cells expressed nestin. (2) In the medium containing 1% FBS, the two NSCs populations could be induced to differentiate into neurons, astrocytes and oligodentrocytes. The percentage of neurons (β-tubulin III-ir) differentiated from brain-derived NSCs decreased rapidly from 11.95 ± 2.5% at passage 2 (P 2) to 1.97 ± 1.16% at passage 5 (P 5). Significant difference was shown between P 2 and P 5 (P <0.01). The percentage of oligodentrocytes (Rip-ir) differentiated from brain-derived NSCs remained mostly unchanged from 8.66 ± 2.93% at P 2 to 9.12 ± 1.13% at P 5. The same differentiation patterns were found in spinal cord-derived NSCs. All these results indicate that both embryonic rat brain- and spinal cord-derived NSCs can expand and proliferate in vitro through multiple passages, and retain the capacity to differentiate into all three major types of cells in the central nervous system.

Original languageEnglish (US)
Pages (from-to)278-283
Number of pages6
JournalSheng li xue bao : [Acta physiologica Sinica]
Volume55
Issue number3
StatePublished - Jun 25 2003
Externally publishedYes

Fingerprint

Neural Stem Cells
Spinal Cord
Brain
Neurons
Nestin
Histocytochemistry
Fibroblast Growth Factor 2
Tubulin
Epidermal Growth Factor
Astrocytes
Fluorescent Antibody Technique
Dissection
Stem Cells
Central Nervous System
Cell Culture Techniques

Keywords

  • Central neural system
  • Embryo
  • Epidermal growth factor
  • Fibroblast growth factor-2
  • Neural stem cell

ASJC Scopus subject areas

  • Physiology

Cite this

Isolation and cultivation of neural stem cells from the embryonic rat brain and spinal cord. / Fu, Sai Li; Ma, Zheng Wen; Yin, Lan; Lu, Pei Hua; Xu, Xiao-Ming.

In: Sheng li xue bao : [Acta physiologica Sinica], Vol. 55, No. 3, 25.06.2003, p. 278-283.

Research output: Contribution to journalArticle

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