Isolation and developmental expression of two nuclear receptors, MHR4 and βFTZ-F1, in the tobacco hornworm, Manduca sexta

John Weller, Guan Cheng Sun, Baohua Zhou, Que Lan, Kiyoshi Hiruma, Lynn M. Riddiford

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The cDNAs for two members of the nuclear receptor superfamily were isolated from the tobacco hornworm, Manduca sexta. The deduced amino acid sequence of MHR4 shows 93-95% identity in the DNA-binding domain and the first portion of the hinge (D) region with the germ cell nuclear factor (GCNF)-related factors (GRFs) of the silkworm, Bombyx mori, and the mealworm, Tenebrio molitor, and with a genomic sequence from the fruit fly, Drosophila melanogaster. Northern blot hybridization showed that a 7.5 kb MHR4 mRNA appeared in Manduca abdominal epidermis just as the ecdysteroid titer began to decline during the larval molt, disappeared about 12 h later, then transiently reappeared shortly before larval ecdysis. During the pupal and adult molts, a similar pattern of expression was seen (the very end of the adult molt was not studied). At peak times of expression in the epidermis, MHR4 mRNA was also present in fat body and the central nervous system (CNS). The deduced amino acid sequence of Manduca FTZ-F1 is 100% and 96% identical to that of B. mori and Drosophila βFTZ-F1, respectively, in the DNA-binding domain and the adjacent hinge region including the FTZ-F1 box. Northern blot analysis showed that the >9.5 kb βFTZ-F1 mRNA appeared in Manduca epidermis during the decline of the ecdysteroid titer in the larval, pupal and adult molts as the first peak of MHR4 mRNA declined, then it disappeared in the larval and pupal molts before the second peak of MHR4 appeared. βFTZ-F1 mRNA was also found in fat body and the CNS at the time of peak expression in the epidermis during the larval and pupal molts. Both MHR4 and βFTZ-F1 mRNAs were found in the testis during the onset of spermatogenesis in the prepupal period.

Original languageEnglish (US)
Pages (from-to)827-837
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume31
Issue number8
DOIs
StatePublished - Jun 22 2001
Externally publishedYes

Fingerprint

Manduca
Tobacco
Manduca sexta
Cytoplasmic and Nuclear Receptors
molting
epidermis (animal)
Epidermis
Messenger RNA
receptors
Bombyx
Tenebrio
Ecdysteroids
Fat Body
DNA-binding domains
ecdysteroids
Neurology
fat body
Hinges
Bombyx mori
Northern blotting

Keywords

  • βFTZ-F1
  • Ecdysone regulation
  • Epidermis
  • Manduca sexta
  • MHR4
  • Nuclear receptors

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Isolation and developmental expression of two nuclear receptors, MHR4 and βFTZ-F1, in the tobacco hornworm, Manduca sexta. / Weller, John; Sun, Guan Cheng; Zhou, Baohua; Lan, Que; Hiruma, Kiyoshi; Riddiford, Lynn M.

In: Insect Biochemistry and Molecular Biology, Vol. 31, No. 8, 22.06.2001, p. 827-837.

Research output: Contribution to journalArticle

Weller, John ; Sun, Guan Cheng ; Zhou, Baohua ; Lan, Que ; Hiruma, Kiyoshi ; Riddiford, Lynn M. / Isolation and developmental expression of two nuclear receptors, MHR4 and βFTZ-F1, in the tobacco hornworm, Manduca sexta. In: Insect Biochemistry and Molecular Biology. 2001 ; Vol. 31, No. 8. pp. 827-837.
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