Isolation and identification of murine bone marrow-derived macrophages and osteomacs from neonatal and adult mice

Joydeep Ghosh, Safa F. Mohamad, Edward F. Srour

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Hematopoietic stem cells (HSCs) are regulated by multiple components of the hematopoietic niche, including bone marrow-derived macrophages and osteomacs. However, both macrophages and osteomacs are phenotypically similar. Thus, specific phenotypic markers are required to differentially identify the effects of osteomacs and bone marrow macrophages on different physiological processes, including hematopoiesis and bone remodeling. Here, we describe a protocol for isolation of murine bone marrow-derived macrophages and osteomacs from neonatal and adult mice and subsequent identification by multi-parametric flow cytometry using an 8-color antibody panel.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages181-193
Number of pages13
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2002
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Bone digestion
  • Bone-marrow macrophages
  • CD166
  • Hematopoietic niche
  • Multiparameter flow cytometry
  • Osteomacs

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ghosh, J., Mohamad, S. F., & Srour, E. F. (2019). Isolation and identification of murine bone marrow-derived macrophages and osteomacs from neonatal and adult mice. In Methods in Molecular Biology (pp. 181-193). (Methods in Molecular Biology; Vol. 2002). Humana Press Inc.. https://doi.org/10.1007/7651_2018_196