Isolation and phenotypic and functional characterization of cells from Peyer's patches in the dog

Harm HogenEsch, Peter J. Felsburg

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cells were isolated from canine Peyer's patches (PPs) and their phenotype and capacity to secrete immunoglobulins in vitro were determined. Cells isolated from duodenal and jejunal PPs of adult dogs consisted of 91.4% lymphocytes and 1.6% macrophages with 55.4% mIg+-cells and 35.6% Thy-1+-cells. In vitro IgA secretion by pokeweed mitogen (PWM)-stimulated PP cells exceeded that by cells from other lymphoid tissues and was specifically increased by concanavalin A, suggesting a role for isotype-specific T-cells. Comparison of duodenal and jejunal (proximal) PPs and the ileal PP revealed that the ileal PP contained fewer T-cells, fewer mIgA+-cells and more mIgM+-cells. Cells from the ileal PP produced very little IgA and IgG, but abundant IgM in vitro. These data suggest that the proximal PPs of dogs are important in the generation of IgA B-cells, similar to PPs of rodents. The ileal PP of dogs may have a function in the early development of the B-cell system of the dog.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume31
Issue number1-2
DOIs
StatePublished - Feb 15 1992
Externally publishedYes

Fingerprint

Peyer's Patches
Peyer's patches
Dogs
dogs
cells
Immunoglobulin A
B-lymphocytes
B-Lymphocytes
T-lymphocytes
T-Lymphocytes
Pokeweed Mitogens
Phytolacca americana
Lymphoid Tissue
concanavalin A
Concanavalin A
immunoglobulins
Immunoglobulin M
early development
Canidae
Immunoglobulins

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Isolation and phenotypic and functional characterization of cells from Peyer's patches in the dog. / HogenEsch, Harm; Felsburg, Peter J.

In: Veterinary Immunology and Immunopathology, Vol. 31, No. 1-2, 15.02.1992, p. 1-10.

Research output: Contribution to journalArticle

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