Isolation, localization, and cardiovascular activity of tachykinins from the stomach of the bowfin Amia calva

D. Waugh, K. E. Groff, B. Platzack, J. H. Youson, Kenneth Olson, J. M. Conlon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The bowfin is an extant representative of an ancient group of ray-tinned fish with evolutionary connections to modern teleosts. A peptide with substance P-like immunoreactivity was isolated from an extract of bowfin stomach and its primary structure was established as Ser-Lys-Ser-His-Gln- Phe-Tyr-Gly-Leu-Met-NH2. This amino acid sequence resembles mammalian substance P only in the COOH-terminal region of the peptide. A second tachykinin with neurokinin A-like immunoreactivity isolated from the extract comprises 23 amino acid residues and shows limited structural similarity to mammalian neuropeptide-γ. A randomly distributed population of cells in the gastric glands of the bowfin were immunostained with an antiserum raised against substance P, but no immunopositive structures were identified in the surface epithelium, lamina propria, or the nerve plexuses of the submucosa. Bolus injections of synthetic bowfin substance P (0.1-10 nmol/kg) into the bulbus arteriosus of unanesthetized bowfin resulted in a significant and dose-dependent rise in vascular resistance and arterial blood pressure (P <0.01) and a fall in cardiac output (P <0.05) without change in heart rate. After 5-10 min, arterial pressure and vascular resistance returned to preinjection levels, but cardiac output significantly (P <0.05) increased over baseline values. The response to the peptide was unaffected by pretreatment of the animals with phentolamine. The study has shown that the stomach of the bowfin synthesizes tachykinins with novel structural features that display cardiovascular activity in this species.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume269
Issue number3 38-3
StatePublished - 1995
Externally publishedYes

Fingerprint

Amia calva
Tachykinins
Substance P
Stomach
stomach
substance P
Cardiac Output
Vascular Resistance
Arterial Pressure
Skates (Fish)
Neurokinin A
Peptides
Phentolamine
peptides
cardiac output
Gastric Mucosa
Neuropeptides
blood vessels
Immune Sera
Amino Acid Sequence

Keywords

  • Amiiformes
  • neuropeptide-γ
  • substance P

ASJC Scopus subject areas

  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Isolation, localization, and cardiovascular activity of tachykinins from the stomach of the bowfin Amia calva. / Waugh, D.; Groff, K. E.; Platzack, B.; Youson, J. H.; Olson, Kenneth; Conlon, J. M.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 269, No. 3 38-3, 1995.

Research output: Contribution to journalArticle

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