Isolation of a novel transposon which carries the Escherichia coli enterotoxin STII gene

Chao-Hung Lee, S. T. Hu, P. J. Swiatek, S. L. Moseley, S. D. Allen, M. So

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The Escherichia coli heat-stable enterotoxin STII gene in P307 is flanked by inverted repeat sequences, suggesting that the STII gene is part of a transposon. To study the transposability, a DNA fragment containing the putative STII transposon has been cloned. Results of transposition assays indicated that the STII gene can transpose from one plasmid to another. The size of the transposon has been determined to be approximately 9 kilobases. The structure and the location of the STII gene in clinical isolates of Escherichia coli have been investigated by restriction enzyme analyses. The structural genes of STII from different clinical isolates appear to be uniform in size, but the flanking sequences are heterogeneous. This result suggests that the STII genes in different isolates are not on the same transposon as observed in P307.

Original languageEnglish
Pages (from-to)615-620
Number of pages6
JournalJournal of Bacteriology
Volume162
Issue number2
StatePublished - 1985

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Escherichia coli
Genes
Inverted Repeat Sequences
Restriction Mapping
heat stable toxin (E coli)
Plasmids
Hot Temperature
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Lee, C-H., Hu, S. T., Swiatek, P. J., Moseley, S. L., Allen, S. D., & So, M. (1985). Isolation of a novel transposon which carries the Escherichia coli enterotoxin STII gene. Journal of Bacteriology, 162(2), 615-620.

Isolation of a novel transposon which carries the Escherichia coli enterotoxin STII gene. / Lee, Chao-Hung; Hu, S. T.; Swiatek, P. J.; Moseley, S. L.; Allen, S. D.; So, M.

In: Journal of Bacteriology, Vol. 162, No. 2, 1985, p. 615-620.

Research output: Contribution to journalArticle

Lee, C-H, Hu, ST, Swiatek, PJ, Moseley, SL, Allen, SD & So, M 1985, 'Isolation of a novel transposon which carries the Escherichia coli enterotoxin STII gene', Journal of Bacteriology, vol. 162, no. 2, pp. 615-620.
Lee, Chao-Hung ; Hu, S. T. ; Swiatek, P. J. ; Moseley, S. L. ; Allen, S. D. ; So, M. / Isolation of a novel transposon which carries the Escherichia coli enterotoxin STII gene. In: Journal of Bacteriology. 1985 ; Vol. 162, No. 2. pp. 615-620.
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