Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

John Chirgwin, Alan E. Przybyla, Raymond J. MacDonald, William J. Rutter

Research output: Contribution to journalArticle

16340 Citations (Scopus)

Abstract

Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was isolated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of α-amylase messenger ribonucleic acid.

Original languageEnglish (US)
Pages (from-to)5294-5299
Number of pages6
JournalBiochemistry
Volume18
Issue number24
StatePublished - 1979
Externally publishedYes

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Ribonucleases
RNA
Rats
Pancreas
Proteins
Mercaptoethanol
Amylases
Sedimentation
Disulfides
Purification
Ethanol
Tissue

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chirgwin, J., Przybyla, A. E., MacDonald, R. J., & Rutter, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry, 18(24), 5294-5299.

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. / Chirgwin, John; Przybyla, Alan E.; MacDonald, Raymond J.; Rutter, William J.

In: Biochemistry, Vol. 18, No. 24, 1979, p. 5294-5299.

Research output: Contribution to journalArticle

Chirgwin, J, Przybyla, AE, MacDonald, RJ & Rutter, WJ 1979, 'Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease', Biochemistry, vol. 18, no. 24, pp. 5294-5299.
Chirgwin, John ; Przybyla, Alan E. ; MacDonald, Raymond J. ; Rutter, William J. / Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. In: Biochemistry. 1979 ; Vol. 18, No. 24. pp. 5294-5299.
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