Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease

John M. Chirgwin, Alan E. Przybyla, Raymond J. MacDonald, William J. Rutter

Research output: Contribution to journalArticlepeer-review

16435 Scopus citations

Abstract

Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was iso lated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of α-amylase messenger ribonucleic acid.

Original languageEnglish (US)
Pages (from-to)5294-5299
Number of pages6
JournalBiochemistry
Volume18
Issue number24
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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