Isolation of osteocytes from mature and aged murine bone

Amber Rath Stern, Lynda Bonewald

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Osteocytes are thought to be the mechanosensors of bone by sensing mechanical loads imposed upon the bone and transmitting these signals to the other bone cells to initiate bone modeling and remodeling. The location of osteocytes deep within bone is ideal for their function. However, this location makes the study of osteocytes in vivo technically difficult. There are several methods for obtaining and culturing primary osteocytes for in vitro experiments and ex vivo observation. In this chapter, several proven methods are discussed including the isolation of avian osteocytes from chicks and osteocytes from calvaria and long bones of young mice. A detailed protocol for the isolation of osteocytes from hypermineralized bone of mature and aged animals is provided.

Original languageEnglish (US)
Title of host publicationOsteoporosis and Osteoarthritis
PublisherSpringer New York
Pages3-10
Number of pages8
ISBN (Electronic)9781493916191
ISBN (Print)9781493916184
DOIs
StatePublished - Oct 20 2014
Externally publishedYes

Fingerprint

Osteocytes
Bone
Bone and Bones
Bone Remodeling
Skull
Animals
Observation

Keywords

  • Age
  • Collagenase
  • Culture
  • Isolation
  • Mice
  • Osteocyte

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Stern, A. R., & Bonewald, L. (2014). Isolation of osteocytes from mature and aged murine bone. In Osteoporosis and Osteoarthritis (pp. 3-10). Springer New York. https://doi.org/10.1007/978-1-4939-1619-1_1

Isolation of osteocytes from mature and aged murine bone. / Stern, Amber Rath; Bonewald, Lynda.

Osteoporosis and Osteoarthritis. Springer New York, 2014. p. 3-10.

Research output: Chapter in Book/Report/Conference proceedingChapter

Stern, AR & Bonewald, L 2014, Isolation of osteocytes from mature and aged murine bone. in Osteoporosis and Osteoarthritis. Springer New York, pp. 3-10. https://doi.org/10.1007/978-1-4939-1619-1_1
Stern AR, Bonewald L. Isolation of osteocytes from mature and aged murine bone. In Osteoporosis and Osteoarthritis. Springer New York. 2014. p. 3-10 https://doi.org/10.1007/978-1-4939-1619-1_1
Stern, Amber Rath ; Bonewald, Lynda. / Isolation of osteocytes from mature and aged murine bone. Osteoporosis and Osteoarthritis. Springer New York, 2014. pp. 3-10
@inbook{02846ead8f7c4d6a962d357cb8c590bf,
title = "Isolation of osteocytes from mature and aged murine bone",
abstract = "Osteocytes are thought to be the mechanosensors of bone by sensing mechanical loads imposed upon the bone and transmitting these signals to the other bone cells to initiate bone modeling and remodeling. The location of osteocytes deep within bone is ideal for their function. However, this location makes the study of osteocytes in vivo technically difficult. There are several methods for obtaining and culturing primary osteocytes for in vitro experiments and ex vivo observation. In this chapter, several proven methods are discussed including the isolation of avian osteocytes from chicks and osteocytes from calvaria and long bones of young mice. A detailed protocol for the isolation of osteocytes from hypermineralized bone of mature and aged animals is provided.",
keywords = "Age, Collagenase, Culture, Isolation, Mice, Osteocyte",
author = "Stern, {Amber Rath} and Lynda Bonewald",
year = "2014",
month = "10",
day = "20",
doi = "10.1007/978-1-4939-1619-1_1",
language = "English (US)",
isbn = "9781493916184",
pages = "3--10",
booktitle = "Osteoporosis and Osteoarthritis",
publisher = "Springer New York",

}

TY - CHAP

T1 - Isolation of osteocytes from mature and aged murine bone

AU - Stern, Amber Rath

AU - Bonewald, Lynda

PY - 2014/10/20

Y1 - 2014/10/20

N2 - Osteocytes are thought to be the mechanosensors of bone by sensing mechanical loads imposed upon the bone and transmitting these signals to the other bone cells to initiate bone modeling and remodeling. The location of osteocytes deep within bone is ideal for their function. However, this location makes the study of osteocytes in vivo technically difficult. There are several methods for obtaining and culturing primary osteocytes for in vitro experiments and ex vivo observation. In this chapter, several proven methods are discussed including the isolation of avian osteocytes from chicks and osteocytes from calvaria and long bones of young mice. A detailed protocol for the isolation of osteocytes from hypermineralized bone of mature and aged animals is provided.

AB - Osteocytes are thought to be the mechanosensors of bone by sensing mechanical loads imposed upon the bone and transmitting these signals to the other bone cells to initiate bone modeling and remodeling. The location of osteocytes deep within bone is ideal for their function. However, this location makes the study of osteocytes in vivo technically difficult. There are several methods for obtaining and culturing primary osteocytes for in vitro experiments and ex vivo observation. In this chapter, several proven methods are discussed including the isolation of avian osteocytes from chicks and osteocytes from calvaria and long bones of young mice. A detailed protocol for the isolation of osteocytes from hypermineralized bone of mature and aged animals is provided.

KW - Age

KW - Collagenase

KW - Culture

KW - Isolation

KW - Mice

KW - Osteocyte

UR - http://www.scopus.com/inward/record.url?scp=84954618871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954618871&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-1619-1_1

DO - 10.1007/978-1-4939-1619-1_1

M3 - Chapter

C2 - 25331038

AN - SCOPUS:84954618871

SN - 9781493916184

SP - 3

EP - 10

BT - Osteoporosis and Osteoarthritis

PB - Springer New York

ER -