Isolation of retinoic acid-repressed genes from P19 embryonal carcinoma cells

Harikrishna Nakshatri, Philippe Bouillet, Poornima Bhat-Nakshatri, Pierre Chambon

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Retinoic acid (RA) plays a critical role in normal development, growth and differentiation by modulating the expression of target genes. Using substractive hybridization cloning, we isolated two cDNAs, whose corresponding mRNAs are repressed upon RA treatment of P19 embryonal carcinoma (EC) cells. The cDNAs correspond to the serine hydroxymethyltransferase (shmt) gene and the early transposon, ETnMG1. RA appears to reduce the stability of ETnMG1 transcript. We also report the sequence of two different isoforms of mouse SHMT. Since SHMT activity is increased when cells are stimulated to proliferate and during the S phase of the cell cycle, we suggest that repression of shmt expression is an important step in RA-induced cell growth arrest and differentiation.

Original languageEnglish (US)
Pages (from-to)79-84
Number of pages6
JournalGene
Volume174
Issue number1
DOIs
StatePublished - Sep 26 1996

Keywords

  • Differentiation
  • Early transposon
  • Gene expression
  • P19 ec cells
  • SHMT

ASJC Scopus subject areas

  • Genetics

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