Isolation of the gene encoding the Drosophila melanogaster homolog of the Saccharomyces cerevisiae GCN2 eIF-2α kinase

DeAnne S. Olsen, Barbara Jordan, Dreeny Chen, Ronald Wek, Douglas R. Cavener

Research output: Contribution to journalArticle

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Abstract

Genomic and cDNA clones homologous to the yeast GCN2 eIF-2α kinase (yGCN2) were isolated from Drosophila melanogaster. The identity of the Drosophila GCN2 (dGCN2) gene is supported by the unique combination of sequence encoding a protein kinase catalytic domain and a domain homologous to histidyl-tRNA synthetase and by the ability of dGCN2 to complement a deletion mutant of the yeast GCN2 gene. Complementation of Δgcn2 in yeast by dGCN2 depends on the presence of the critical regulatory phosphorylation site (serine 51) of eIF-2α. dGCN2 is composed of 10 exons encoding a protein of 1589 amino acids. dGCN2 mRNA is expressed throughout Drosophila development and is particularly abundant at the earliest stages of embryogenesis. The dGCN2 gene was cytogenetically and physically mapped to the right arm of the third chromosome at 100C3 in STS Dm2514. The discovery of GCN2 in higher eukaryotes is somewhat unexpected given the marked differences between the amino acid biosynthetic pathways of yeast vs. Drosophila and other higher eukaryotes. Despite these differences, the presence of GCN2 in Drosophila suggests at least partial conservation from yeast to multicellular organisms of the mechanisms responding to amino acid deprivation.

Original languageEnglish
Pages (from-to)1495-1509
Number of pages15
JournalGenetics
Volume149
Issue number3
StatePublished - 1998

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eIF-2 Kinase
Drosophila melanogaster
Drosophila
Saccharomyces cerevisiae
Genes
Yeasts
Eukaryota
Amino Acids
Histidine-tRNA Ligase
Biosynthetic Pathways
Protein Kinases
Serine
Embryonic Development
Exons
Catalytic Domain
Complementary DNA
Clone Cells
Chromosomes
Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Isolation of the gene encoding the Drosophila melanogaster homolog of the Saccharomyces cerevisiae GCN2 eIF-2α kinase. / Olsen, DeAnne S.; Jordan, Barbara; Chen, Dreeny; Wek, Ronald; Cavener, Douglas R.

In: Genetics, Vol. 149, No. 3, 1998, p. 1495-1509.

Research output: Contribution to journalArticle

Olsen, DeAnne S. ; Jordan, Barbara ; Chen, Dreeny ; Wek, Ronald ; Cavener, Douglas R. / Isolation of the gene encoding the Drosophila melanogaster homolog of the Saccharomyces cerevisiae GCN2 eIF-2α kinase. In: Genetics. 1998 ; Vol. 149, No. 3. pp. 1495-1509.
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