Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10

Jason J. Chen, Deborah J. McNealy, Sorab Dalal, Elliot Androphy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A full-length cDNA clone encoding chaperonin 10 (cpn10) from a HeLa cell cDNA library was isolated. The cDNA is 538 bp in length, contains an ATG codon and a putative polyadenylation signal, and specifies a protein of 102 amino acids. Immunoprecipitation experiment showed that this human cpn10 has an apparent molecular mass of 11 kDa in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Original languageEnglish (US)
Pages (from-to)189-190
Number of pages2
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1219
Issue number1
DOIs
StatePublished - Sep 13 1994
Externally publishedYes

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Chaperonin 10
Sequence Analysis
Complementary DNA
Polyadenylation
Molecular mass
Electrophoresis
Gene Library
HeLa Cells
Immunoprecipitation
Codon
Polyacrylamide Gel Electrophoresis
Clone Cells
Sodium
Amino Acids
Proteins
Experiments

Keywords

  • (Human)
  • Chaperonin
  • Cloning
  • Nucleotide sequence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Isolation, sequence analysis and characterization of a cDNA encoding human chaperonin 10. / Chen, Jason J.; McNealy, Deborah J.; Dalal, Sorab; Androphy, Elliot.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1219, No. 1, 13.09.1994, p. 189-190.

Research output: Contribution to journalArticle

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