Isolationof synaptosomes and synaptic membranes from the cerebral cortex of the rat, monkey, cat and pig

William J. McBride, James G. Perkins, Henry R. Mahler, Walter J. Moore

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Isolation of synaptosomes and synaptic membranes from the cerebral cortex of the rat, monkey, cat and pig was achieved by centrifugation of the crude mitochondrial fraction through a continuous sucrose gradient using high-speed zonal rotors. Electron microscopy and the activity of certain marker enzymes, such as choline acetyltransferase, acetylcholinesterase and (Na+-K+)-activated ouabin-sensitive ATPase, were used to characterize the various gradient fractions. In all four species studied the synaptosomes were found in the region from 31 to 37% sucrose of the gradient of the crude mitochondrial fraction (P2) and a synaptic plasma membrane fraction was found in the region from 24 to 31% sucrose of the gradient of the lysed crude mitochondrial fraction (P2L). In the case of the rat and monkey there appeared to be some resolution of noncholinergic nerve endings from cholinergic endings.

Original languageEnglish
Pages (from-to)975-987
Number of pages13
JournalLife Sciences
Volume11
Issue number20 PART 1
DOIs
StatePublished - Oct 15 1972

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Synaptic Membranes
Synaptosomes
Cerebral Cortex
Haplorhini
Sucrose
Rats
Cats
Swine
Membranes
Choline O-Acetyltransferase
Nerve Endings
Centrifugation
Cell membranes
Acetylcholinesterase
Cholinergic Agents
Electron microscopy
Adenosine Triphosphatases
Electron Microscopy
Rotors
Cell Membrane

ASJC Scopus subject areas

  • Pharmacology

Cite this

Isolationof synaptosomes and synaptic membranes from the cerebral cortex of the rat, monkey, cat and pig. / McBride, William J.; Perkins, James G.; Mahler, Henry R.; Moore, Walter J.

In: Life Sciences, Vol. 11, No. 20 PART 1, 15.10.1972, p. 975-987.

Research output: Contribution to journalArticle

McBride, William J. ; Perkins, James G. ; Mahler, Henry R. ; Moore, Walter J. / Isolationof synaptosomes and synaptic membranes from the cerebral cortex of the rat, monkey, cat and pig. In: Life Sciences. 1972 ; Vol. 11, No. 20 PART 1. pp. 975-987.
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