JP-8 jet fuel exposure alters protein expression in the lung

Matthew G. Drake, Frank Witzmann, Juanita Hyde, Mark L. Witten

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The purpose of this study was to investigate the proteomic mechanisms of Jet Propulsion-8 (JP-8) toxicity in the lung, specifically relating to lung epithelial cell apoptosis and edema. Male Swiss-Webster mice were exposed to 1 h/day aerosolized JP-8 jet fuel at concentrations of 250, 1000, and 2500 mg/m3 for 7 days. Lung cytosol and whole lung samples were solubilized, separated via large scale, high-resolution two-dimensional electrophoresis, and processed for analysis. Significant quantitative differences in lung protein expression were found as a result of JP-8 exposure. At 250 mg/m3 JP-8 concentration, 31 proteins exhibited increased expression, while 10 showed decreased expression. At 1000 mg/m3 exposure levels, 21 lung proteins exhibited increased expression and 99 demonstrated decreased expression. At 2500 mg/m3, 30 exhibited increased expression, while 135 showed decreased expression. Several of the proteins were identified by peptide mass fingerprinting, and were found to relate to cell structure, cell proliferation, protein repair, and apoptosis. These data demonstrate the significant stress JP-8 jet fuel puts on lung epithelium. Furthermore, there was a decrease in α1-anti-trypsin expression suggesting that JP-8 jet fuel exposure may have implications for the development of pulmonary disorders.

Original languageEnglish
Pages (from-to)199-210
Number of pages12
JournalToxicology
Volume191
Issue number2-3
DOIs
StatePublished - Sep 30 2003

Fingerprint

Jet fuel
Propulsion
Lung
Proteins
Apoptosis
Cell proliferation
Electrophoresis
Trypsin
Peptide Mapping
Toxicity
Repair
Cytosol
Proteomics
Edema
Peptides
Epithelium
Epithelial Cells
Cell Proliferation

Keywords

  • JP-8 jet fuel
  • Lung
  • Mouse
  • Proteomics
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Toxicology

Cite this

JP-8 jet fuel exposure alters protein expression in the lung. / Drake, Matthew G.; Witzmann, Frank; Hyde, Juanita; Witten, Mark L.

In: Toxicology, Vol. 191, No. 2-3, 30.09.2003, p. 199-210.

Research output: Contribution to journalArticle

Drake, Matthew G. ; Witzmann, Frank ; Hyde, Juanita ; Witten, Mark L. / JP-8 jet fuel exposure alters protein expression in the lung. In: Toxicology. 2003 ; Vol. 191, No. 2-3. pp. 199-210.
@article{4ce32fbbd1424372ae34eee9fd556ed7,
title = "JP-8 jet fuel exposure alters protein expression in the lung",
abstract = "The purpose of this study was to investigate the proteomic mechanisms of Jet Propulsion-8 (JP-8) toxicity in the lung, specifically relating to lung epithelial cell apoptosis and edema. Male Swiss-Webster mice were exposed to 1 h/day aerosolized JP-8 jet fuel at concentrations of 250, 1000, and 2500 mg/m3 for 7 days. Lung cytosol and whole lung samples were solubilized, separated via large scale, high-resolution two-dimensional electrophoresis, and processed for analysis. Significant quantitative differences in lung protein expression were found as a result of JP-8 exposure. At 250 mg/m3 JP-8 concentration, 31 proteins exhibited increased expression, while 10 showed decreased expression. At 1000 mg/m3 exposure levels, 21 lung proteins exhibited increased expression and 99 demonstrated decreased expression. At 2500 mg/m3, 30 exhibited increased expression, while 135 showed decreased expression. Several of the proteins were identified by peptide mass fingerprinting, and were found to relate to cell structure, cell proliferation, protein repair, and apoptosis. These data demonstrate the significant stress JP-8 jet fuel puts on lung epithelium. Furthermore, there was a decrease in α1-anti-trypsin expression suggesting that JP-8 jet fuel exposure may have implications for the development of pulmonary disorders.",
keywords = "JP-8 jet fuel, Lung, Mouse, Proteomics, Two-dimensional electrophoresis",
author = "Drake, {Matthew G.} and Frank Witzmann and Juanita Hyde and Witten, {Mark L.}",
year = "2003",
month = "9",
day = "30",
doi = "10.1016/S0300-483X(03)00251-8",
language = "English",
volume = "191",
pages = "199--210",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - JP-8 jet fuel exposure alters protein expression in the lung

AU - Drake, Matthew G.

AU - Witzmann, Frank

AU - Hyde, Juanita

AU - Witten, Mark L.

PY - 2003/9/30

Y1 - 2003/9/30

N2 - The purpose of this study was to investigate the proteomic mechanisms of Jet Propulsion-8 (JP-8) toxicity in the lung, specifically relating to lung epithelial cell apoptosis and edema. Male Swiss-Webster mice were exposed to 1 h/day aerosolized JP-8 jet fuel at concentrations of 250, 1000, and 2500 mg/m3 for 7 days. Lung cytosol and whole lung samples were solubilized, separated via large scale, high-resolution two-dimensional electrophoresis, and processed for analysis. Significant quantitative differences in lung protein expression were found as a result of JP-8 exposure. At 250 mg/m3 JP-8 concentration, 31 proteins exhibited increased expression, while 10 showed decreased expression. At 1000 mg/m3 exposure levels, 21 lung proteins exhibited increased expression and 99 demonstrated decreased expression. At 2500 mg/m3, 30 exhibited increased expression, while 135 showed decreased expression. Several of the proteins were identified by peptide mass fingerprinting, and were found to relate to cell structure, cell proliferation, protein repair, and apoptosis. These data demonstrate the significant stress JP-8 jet fuel puts on lung epithelium. Furthermore, there was a decrease in α1-anti-trypsin expression suggesting that JP-8 jet fuel exposure may have implications for the development of pulmonary disorders.

AB - The purpose of this study was to investigate the proteomic mechanisms of Jet Propulsion-8 (JP-8) toxicity in the lung, specifically relating to lung epithelial cell apoptosis and edema. Male Swiss-Webster mice were exposed to 1 h/day aerosolized JP-8 jet fuel at concentrations of 250, 1000, and 2500 mg/m3 for 7 days. Lung cytosol and whole lung samples were solubilized, separated via large scale, high-resolution two-dimensional electrophoresis, and processed for analysis. Significant quantitative differences in lung protein expression were found as a result of JP-8 exposure. At 250 mg/m3 JP-8 concentration, 31 proteins exhibited increased expression, while 10 showed decreased expression. At 1000 mg/m3 exposure levels, 21 lung proteins exhibited increased expression and 99 demonstrated decreased expression. At 2500 mg/m3, 30 exhibited increased expression, while 135 showed decreased expression. Several of the proteins were identified by peptide mass fingerprinting, and were found to relate to cell structure, cell proliferation, protein repair, and apoptosis. These data demonstrate the significant stress JP-8 jet fuel puts on lung epithelium. Furthermore, there was a decrease in α1-anti-trypsin expression suggesting that JP-8 jet fuel exposure may have implications for the development of pulmonary disorders.

KW - JP-8 jet fuel

KW - Lung

KW - Mouse

KW - Proteomics

KW - Two-dimensional electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=0142024970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142024970&partnerID=8YFLogxK

U2 - 10.1016/S0300-483X(03)00251-8

DO - 10.1016/S0300-483X(03)00251-8

M3 - Article

C2 - 12965123

AN - SCOPUS:0142024970

VL - 191

SP - 199

EP - 210

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 2-3

ER -