Kappa light chain-associated Fanconi's syndrome: Molecular analysis of monoclonal immunoglobulin light chains from patients with and without intracellular crystals

Sophie Déret, Luc Denoroy, Marc Lamarine, Ruben Vidal, Béatrice Mougenot, Blas Frangione, Fred J. Stevens, Pierre M. Ronco, Pierre Aucouturier

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Plasma cell dyscrasias may be responsible for Fanconi's syndrome, due to the toxicity of a free monoclonal kappa light chain toward kidney proximal tubules. Eight cases of Fanconi's syndrome were analyzed. We compared the structures of VκI variability subgroup V domains from five cases of Fanconi's syndrome and one myeloma without renal involvement. Among Fanconi cases, four putative structures were obtained after molecular modeling by homology, and the other had previously been refined by X-ray crystallography. The complete sequences of one VκI, one VκIII and N-terminal sequences of two VκI light chains, from patients with different forms of Fanconi's syndrome, were compared with four previously studied sequences. All three kappa chains responsible for a 'classical' form with intralysosomal crystals and a low mass myeloma, were encoded by the LCO2/O12 germline gene and had an unusual non-polar residue exposed to the solvent in the CDR-L1 loop. Of both VκI light chains from patients with Fanconi's syndrome without intracellular crystals, one derived from LCO2/O12 and the other from LCO8/O18 gene. Another feature that could be related to non-crystallization was the absence of accessible side chains in the CDR-L3 loop which is known to be implicated in dimer formation.

Original languageEnglish (US)
Pages (from-to)363-369
Number of pages7
JournalProtein Engineering
Volume12
Issue number4
StatePublished - May 17 1999

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Keywords

  • Bence-Jones protein
  • Human
  • Kidney
  • Myeloma
  • Protein structure

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Déret, S., Denoroy, L., Lamarine, M., Vidal, R., Mougenot, B., Frangione, B., Stevens, F. J., Ronco, P. M., & Aucouturier, P. (1999). Kappa light chain-associated Fanconi's syndrome: Molecular analysis of monoclonal immunoglobulin light chains from patients with and without intracellular crystals. Protein Engineering, 12(4), 363-369.