Ketone body and acetate formation from oleate by isolated rat testicular cells

Emily A. Yount, Robert Harris

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Isolated rat testicular cells oxidize oleate to CO2, acetate, ketone bodies, and other compounds. Carnitine inhibited acetate, but not CO2 and ketone body, production from oleate by isolated adult rat testicular cells. Lactate inhibited ketone body production with glucose as substrate in the absence, but not the presence, of added oleate. The effects of 2-chloropropionate and dichloroacetate on carbohydrate and lipid metabolism in these cells were not consistent with significant activation of the pyruvate dehydrogenase complex. Rates of oleate oxidation to CO2 were greater in cells prepared from immature rats than in cells from adults. Compared to cells from adults, cells from young rats oxidized 3-hydroxybutyrate more rapidly and accumulated less acetoacetate and 3-hydroxybutyrate. In cells from young, but not adult rats, oleate inhibited oxidation of [U-14C]glucose to 14CO2. Although the role of lipid as a fuel for adult rat testes is uncertain, lipid may well be an important source of energy for the prepubertal rat testis.

Original languageEnglish
Pages (from-to)503-511
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume217
Issue number2
DOIs
StatePublished - 1982

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Ketone Bodies
Oleic Acid
Rats
Acetates
3-Hydroxybutyric Acid
Testis
Young Adult
Pyruvate Dehydrogenase Complex
Lipids
Glucose
Oxidation
Carnitine
Carbohydrate Metabolism
Lipid Metabolism
Lactic Acid
Chemical activation
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ketone body and acetate formation from oleate by isolated rat testicular cells. / Yount, Emily A.; Harris, Robert.

In: Archives of Biochemistry and Biophysics, Vol. 217, No. 2, 1982, p. 503-511.

Research output: Contribution to journalArticle

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