Isolated rat testicular cells oxidize oleate to CO2, acetate, ketone bodies, and other compounds. Carnitine inhibited acetate, but not CO2 and ketone body, production from oleate by isolated adult rat testicular cells. Lactate inhibited ketone body production with glucose as substrate in the absence, but not the presence, of added oleate. The effects of 2-chloropropionate and dichloroacetate on carbohydrate and lipid metabolism in these cells were not consistent with significant activation of the pyruvate dehydrogenase complex. Rates of oleate oxidation to CO2 were greater in cells prepared from immature rats than in cells from adults. Compared to cells from adults, cells from young rats oxidized 3-hydroxybutyrate more rapidly and accumulated less acetoacetate and 3-hydroxybutyrate. In cells from young, but not adult rats, oleate inhibited oxidation of [U-14C]glucose to 14CO2. Although the role of lipid as a fuel for adult rat testes is uncertain, lipid may well be an important source of energy for the prepubertal rat testis.
ASJC Scopus subject areas
- Molecular Biology