KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers

Timothy Corson, Annie Huang, Ming Sound Tsao, Brenda L. Gallie

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Gain of chromosome 1q31-1q32 is seen in >50% of retinoblastoma and is common in other tumors. To define the minimal 1q region of gain, we determined genomic copy number by quantitative multiplex PCR of 14 sequence tagged sites (STSs) spanning 1q25.3-1q41. The most frequently gained STS at 1q32.1 (71%; 39 of 55 retinoblastoma) defined a 3.06 Mbp minimal region of gain between flanking markers, containing 14 genes. Of these, only KIF14, a putative chromokinesin, was overexpressed in various cancers by real-time RT-PCR. KIF14 mRNA was expressed in 20/22 retinoblastoma samples 100-1000-fold higher than in retina (t-test P = 0.00002); cell lines (n = 10) had higher levels than tumors (n = 12) (P = 0.009). KIF14 protein was overexpressed in retinoblastoma tumors and breast cancer cell lines by immunoblot. KIF14 was expressed in 4/4 breast cancer cell lines 31-92-fold higher than in normal breast tissue, in 5/5 medulloblastoma cell lines 22-79-fold higher than in fetal brain, and in 10/22 primary lung tumors 3-34-fold higher than in normal lung. Patients with lung tumors that overexpress KIf14 showed a trend toward decreased survival. KIF14 may thus be important in oncogenesis, and has promise as a prognostic indicator and therapeutic target.

Original languageEnglish (US)
Pages (from-to)4741-4753
Number of pages13
JournalOncogene
Volume24
Issue number30
DOIs
StatePublished - Jul 14 2005
Externally publishedYes

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Oncogenes
Retinoblastoma
Sequence Tagged Sites
Cell Line
Neoplasms
Lung
Breast Neoplasms
Medulloblastoma
Multiplex Polymerase Chain Reaction
Retina
Real-Time Polymerase Chain Reaction
Carcinogenesis
Breast
Chromosomes
Messenger RNA
Survival
Brain
Genes
Proteins

Keywords

  • Breast cancer
  • Chromosome 1
  • Kinesin
  • Lung cancer
  • Medulloblastoma
  • Retinoblastoma

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers. / Corson, Timothy; Huang, Annie; Tsao, Ming Sound; Gallie, Brenda L.

In: Oncogene, Vol. 24, No. 30, 14.07.2005, p. 4741-4753.

Research output: Contribution to journalArticle

Corson, Timothy ; Huang, Annie ; Tsao, Ming Sound ; Gallie, Brenda L. / KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers. In: Oncogene. 2005 ; Vol. 24, No. 30. pp. 4741-4753.
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abstract = "Gain of chromosome 1q31-1q32 is seen in >50{\%} of retinoblastoma and is common in other tumors. To define the minimal 1q region of gain, we determined genomic copy number by quantitative multiplex PCR of 14 sequence tagged sites (STSs) spanning 1q25.3-1q41. The most frequently gained STS at 1q32.1 (71{\%}; 39 of 55 retinoblastoma) defined a 3.06 Mbp minimal region of gain between flanking markers, containing 14 genes. Of these, only KIF14, a putative chromokinesin, was overexpressed in various cancers by real-time RT-PCR. KIF14 mRNA was expressed in 20/22 retinoblastoma samples 100-1000-fold higher than in retina (t-test P = 0.00002); cell lines (n = 10) had higher levels than tumors (n = 12) (P = 0.009). KIF14 protein was overexpressed in retinoblastoma tumors and breast cancer cell lines by immunoblot. KIF14 was expressed in 4/4 breast cancer cell lines 31-92-fold higher than in normal breast tissue, in 5/5 medulloblastoma cell lines 22-79-fold higher than in fetal brain, and in 10/22 primary lung tumors 3-34-fold higher than in normal lung. Patients with lung tumors that overexpress KIf14 showed a trend toward decreased survival. KIF14 may thus be important in oncogenesis, and has promise as a prognostic indicator and therapeutic target.",
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