Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules

Shang Cai, Lesley N. Weaver, Stephanie C. Ems-McClung, Claire Walczak

Research output: Contribution to journalArticle

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Abstract

Kinesin-14 family proteins are minus-end directed motors that cross-link microtubules and play key roles during spindle assembly. We showed previously that the Xenopus Kinesin-14 XCTK2 is regulated by Ran via the association of a bipartite NLS in the tail of XCTK2 with importin α/β which regulates its ability to cross-link microtubules during spindle formation. Here we show that mutation of the nuclear localization signal (NLS) of human Kinesin-14 HSET caused an accumulation of HSET in the cytoplasm, which resulted in strong microtubule bundling. HSET overexpression in HeLa cells resulted in longer spindles, similar to what was seen with NLS mutants of XCTK2 in extracts, suggesting that Kinesin-14 proteins play similar roles in extracts and in somatic cells. Conversely, HSET knockdown by RNAi resulted in shorter spindles but did not affect pole formation. The change in spindle length was not dependent on K-fibers, as elimination of the K-fiber by Nuf2 RNAi resulted in an increase in spindle length that was partially rescued by co-RNAi of HSET. However, these changes in spindle length did require microtubule sliding, as overexpression of an HSET mutant that had its sliding activity uncoupled from its ATPase activity resulted in cells with spindle lengths shorter than cells overexpressing wild-type HSET. Our results are consistent with a model in which Ran regulates the association of Kinesin-14s with importin α/β to prevent aberrant cross-linking and bundling of microtubules by sequestering Kinesin- 14s in the nucleus during interphase. Kinesin-14s act during mitosis to cross-link and slide between parallel microtubules to regulate spindle length.

Original languageEnglish
Pages (from-to)1348-1359
Number of pages12
JournalMolecular Biology of the Cell
Volume20
Issue number5
DOIs
StatePublished - Mar 1 2009

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Kinesin
Microtubules
Nuclear Localization Signals
RNA Interference
Karyopherins
Proteins
Interphase
Xenopus
HeLa Cells
Mitosis
Adenosine Triphosphatases
Tail
Cytoplasm
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules. / Cai, Shang; Weaver, Lesley N.; Ems-McClung, Stephanie C.; Walczak, Claire.

In: Molecular Biology of the Cell, Vol. 20, No. 5, 01.03.2009, p. 1348-1359.

Research output: Contribution to journalArticle

Cai, Shang ; Weaver, Lesley N. ; Ems-McClung, Stephanie C. ; Walczak, Claire. / Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 5. pp. 1348-1359.
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