Kinetics and type of immune response following intranasal and subcutaneous immunisation of calves

M. C. Rebelatto, L. Siger, Harm HogenEsch

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Protection of animals against respiratory infections has long been known to depend on respiratory mucosal immunity. However, few studies have been reported on the immune response following intranasal (i.n.) immunisation with non-living, soluble antigens. This study determined the kinetics of the humoral and cellular immune responses in calves after i.n. immunisation with Limulus haemocyanin (LH) with cholera toxin adjuvant, or subcutaneous (s.c.) immunisation with LH in incomplete Freund's adjuvant. A proliferative response of peripheral blood mononuclear cells cultured in vitro with LH was observed in animals immunised 7-10 days after i.n. and s.c. immunisations with no significant differences between the two immunised groups. LH-specific antibody was present in the serum of animals immunised s.c. (IgM, IgG1 and IgG2) and i.n. (IgA). Although significant IgA responses were observed, i.n. immunisations in cattle with soluble protein antigens and cholera toxin as an adjuvant did not induce a strong systemic immune response.

Original languageEnglish (US)
Pages (from-to)9-15
Number of pages7
JournalResearch in Veterinary Science
Volume71
Issue number1
StatePublished - 2001
Externally publishedYes

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Limulus
Horseshoe Crabs
Hemocyanin
Immunization
immunization
immune response
calves
kinetics
adjuvants
cholera toxin
Cholera Toxin
Immunoglobulin A
Immunoglobulin G
antigens
mucosal immunity
Antigens
Mucosal Immunity
animals
Humoral Immunity
mononuclear leukocytes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Kinetics and type of immune response following intranasal and subcutaneous immunisation of calves. / Rebelatto, M. C.; Siger, L.; HogenEsch, Harm.

In: Research in Veterinary Science, Vol. 71, No. 1, 2001, p. 9-15.

Research output: Contribution to journalArticle

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