Kinetics of copper-PTSM in isolated hearts: A novel tracer for measuring blood flow with positron emission tomography

M. E. Shelton, Mark Green, C. J. Mathias, M. J. Welch, S. R. Bergmann

Research output: Contribution to journalArticle

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Abstract

Copper(II) pyruvaldehyde bis(N4-methylthiosemicarbazone) ([Cu]PTSM) has shown potential as a flow tracer and can be labeled with the generator-produced positron emitting radionuclide 62Cu as well as with other copper radioisotopes. The define the myocardial handling of [Cu]PTSM, the externally detected single pass extraction and retention of [67Cu]PTSM was characterized after bolus administration in 12 isolated rabbit hearts perfused with erythrocyte-enriched modified Krebs-Henseleit buffer which permitted physiologic flow rates. The myocardial residual (extraction) fraction at control flow rates (~1.5 ml/g/min) was 45 ± 7 (s.d.)% (n = 12), and was invariate with ischemia (flow = 0.15 ml/g/min, n = 4), hyperemia (flow = 3 ml/g/min, n = 4) or with hypoxia induced by perfusion at control flow rates with hypoxic buffer (n = 4) (residual fraction 45 ± 20, 43 ± 8, and 49 ± 8%, respectively, p = N.S.). Once extracted, the tracer was retained with a biologic t( 1/2 ) of >3600 min in all groups. The high single-pass extraction, which is not influenced by flow, and the prolonged retention of this tracer under diverse conditions indicate that [Cu]PTSM could be a useful tracer for measuring blood flow with positron emission tomography.

Original languageEnglish (US)
Pages (from-to)1843-1847
Number of pages5
JournalJournal of Nuclear Medicine
Volume30
Issue number11
StatePublished - 1989
Externally publishedYes

Fingerprint

Positron-Emission Tomography
Copper
Copper Radioisotopes
Pyruvaldehyde
Hyperemia
Radioisotopes
Buffers
Ischemia
Perfusion
Erythrocytes
Electrons
Rabbits
copper pyruvaldehyde bis(N(4)-methylthiosemicarbazone) complex
Hypoxia
Krebs-Henseleit solution

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Kinetics of copper-PTSM in isolated hearts : A novel tracer for measuring blood flow with positron emission tomography. / Shelton, M. E.; Green, Mark; Mathias, C. J.; Welch, M. J.; Bergmann, S. R.

In: Journal of Nuclear Medicine, Vol. 30, No. 11, 1989, p. 1843-1847.

Research output: Contribution to journalArticle

Shelton, M. E. ; Green, Mark ; Mathias, C. J. ; Welch, M. J. ; Bergmann, S. R. / Kinetics of copper-PTSM in isolated hearts : A novel tracer for measuring blood flow with positron emission tomography. In: Journal of Nuclear Medicine. 1989 ; Vol. 30, No. 11. pp. 1843-1847.
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