Known genetic susceptibility factors for chronic pancreatitis in patients of European ancestry are rare in patients of African ancestry

Anna Evans Phillips, Jessica LaRusch, Phil Greer, Judah Abberbock, Samer Alkaade, Stephen T. Amann, Michelle A. Anderson, John Baillie, Peter A. Banks, Randall E. Brand, Darwin Conwell, Gregory A. Coté, Christopher E. Forsmark, Timothy B. Gardner, Andres Gelrud, Nalini Guda, Michele Lewis, Mary E. Money, Thiruvengadam Muniraj, Bimaljit S. SandhuStuart Sherman, Vikesh K. Singh, Adam Slivka, Gong Tang, C. Mel Wilcox, David C. Whitcomb, Dhiraj Yadav

Research output: Contribution to journalArticle

Abstract

Background: Multiple pathogenic genetic variants are associated with pancreatitis in patients of European (EA) and Asian ancestries, but studies on patients of African ancestry (AA) are lacking. We evaluated the prevalence of known genetic variations in African-American subjects in the US. Methods: We studied prospectively enrolled controls (n = 238) and patients with chronic (CP) (n = 232) or recurrent acute pancreatitis (RAP) (n = 45) in the NAPS2 studies from 2000-2014 of self-identified AA. Demographic and phenotypic information was obtained from structured questionnaires. Ancestry and admixture were evaluated by principal component analysis (PCA). Genotyping was performed for pathogenic genetic variants in PRSS1, SPINK1, CFTR and CTRC. Prevalence of disease-associated variants in NAPS2 subjects of AA and EA was compared. Results: When compared with CP subjects of EA (n = 862), prevalence of established pathogenic genetic variants was infrequent in AA patients with CP, overall (29 vs. 8.19%, OR 4.60, 95% CI 2.74–7.74, p < 0.001), and after stratification by alcohol etiology (p < 0.001). On PCA, AA cases were more heterogeneous but distinct from EA subjects; no difference was observed between AA subjects with and without CP-associated variants. Of 19 A A patients with CP who had pathogenic genetic variants, 2 had variants in PRSS1 (R122H, R122C), 4 in SPINK1 (all N34S heterozygotes), 12 in CFTR (2 CFTRsev, 9 CFTRBD, 1 compound heterozygote with CFTRsev and CFTRBD), and 1 in CTRC (R254W). Conclusion: Pathogenic genetic variants reported in EA patients are significantly less common in AA patients. Further studies are needed to determine the complex risk factors for AA subjects with pancreatitis.

Original languageEnglish (US)
Pages (from-to)528-535
Number of pages8
JournalPancreatology
Volume18
Issue number5
DOIs
StatePublished - Jul 1 2018

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Chronic Pancreatitis
Genetic Predisposition to Disease
Pancreatitis
Heterozygote
Principal Component Analysis
African Americans
Alcohols
Demography

Keywords

  • African ancestry
  • African-American
  • Alcohol
  • Blacks
  • Genetics
  • Pancreatitis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Gastroenterology

Cite this

Known genetic susceptibility factors for chronic pancreatitis in patients of European ancestry are rare in patients of African ancestry. / Phillips, Anna Evans; LaRusch, Jessica; Greer, Phil; Abberbock, Judah; Alkaade, Samer; Amann, Stephen T.; Anderson, Michelle A.; Baillie, John; Banks, Peter A.; Brand, Randall E.; Conwell, Darwin; Coté, Gregory A.; Forsmark, Christopher E.; Gardner, Timothy B.; Gelrud, Andres; Guda, Nalini; Lewis, Michele; Money, Mary E.; Muniraj, Thiruvengadam; Sandhu, Bimaljit S.; Sherman, Stuart; Singh, Vikesh K.; Slivka, Adam; Tang, Gong; Wilcox, C. Mel; Whitcomb, David C.; Yadav, Dhiraj.

In: Pancreatology, Vol. 18, No. 5, 01.07.2018, p. 528-535.

Research output: Contribution to journalArticle

Phillips, AE, LaRusch, J, Greer, P, Abberbock, J, Alkaade, S, Amann, ST, Anderson, MA, Baillie, J, Banks, PA, Brand, RE, Conwell, D, Coté, GA, Forsmark, CE, Gardner, TB, Gelrud, A, Guda, N, Lewis, M, Money, ME, Muniraj, T, Sandhu, BS, Sherman, S, Singh, VK, Slivka, A, Tang, G, Wilcox, CM, Whitcomb, DC & Yadav, D 2018, 'Known genetic susceptibility factors for chronic pancreatitis in patients of European ancestry are rare in patients of African ancestry', Pancreatology, vol. 18, no. 5, pp. 528-535. https://doi.org/10.1016/j.pan.2018.05.482
Phillips, Anna Evans ; LaRusch, Jessica ; Greer, Phil ; Abberbock, Judah ; Alkaade, Samer ; Amann, Stephen T. ; Anderson, Michelle A. ; Baillie, John ; Banks, Peter A. ; Brand, Randall E. ; Conwell, Darwin ; Coté, Gregory A. ; Forsmark, Christopher E. ; Gardner, Timothy B. ; Gelrud, Andres ; Guda, Nalini ; Lewis, Michele ; Money, Mary E. ; Muniraj, Thiruvengadam ; Sandhu, Bimaljit S. ; Sherman, Stuart ; Singh, Vikesh K. ; Slivka, Adam ; Tang, Gong ; Wilcox, C. Mel ; Whitcomb, David C. ; Yadav, Dhiraj. / Known genetic susceptibility factors for chronic pancreatitis in patients of European ancestry are rare in patients of African ancestry. In: Pancreatology. 2018 ; Vol. 18, No. 5. pp. 528-535.
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abstract = "Background: Multiple pathogenic genetic variants are associated with pancreatitis in patients of European (EA) and Asian ancestries, but studies on patients of African ancestry (AA) are lacking. We evaluated the prevalence of known genetic variations in African-American subjects in the US. Methods: We studied prospectively enrolled controls (n = 238) and patients with chronic (CP) (n = 232) or recurrent acute pancreatitis (RAP) (n = 45) in the NAPS2 studies from 2000-2014 of self-identified AA. Demographic and phenotypic information was obtained from structured questionnaires. Ancestry and admixture were evaluated by principal component analysis (PCA). Genotyping was performed for pathogenic genetic variants in PRSS1, SPINK1, CFTR and CTRC. Prevalence of disease-associated variants in NAPS2 subjects of AA and EA was compared. Results: When compared with CP subjects of EA (n = 862), prevalence of established pathogenic genetic variants was infrequent in AA patients with CP, overall (29 vs. 8.19{\%}, OR 4.60, 95{\%} CI 2.74–7.74, p < 0.001), and after stratification by alcohol etiology (p < 0.001). On PCA, AA cases were more heterogeneous but distinct from EA subjects; no difference was observed between AA subjects with and without CP-associated variants. Of 19 A A patients with CP who had pathogenic genetic variants, 2 had variants in PRSS1 (R122H, R122C), 4 in SPINK1 (all N34S heterozygotes), 12 in CFTR (2 CFTRsev, 9 CFTRBD, 1 compound heterozygote with CFTRsev and CFTRBD), and 1 in CTRC (R254W). Conclusion: Pathogenic genetic variants reported in EA patients are significantly less common in AA patients. Further studies are needed to determine the complex risk factors for AA subjects with pancreatitis.",
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T1 - Known genetic susceptibility factors for chronic pancreatitis in patients of European ancestry are rare in patients of African ancestry

AU - Phillips, Anna Evans

AU - LaRusch, Jessica

AU - Greer, Phil

AU - Abberbock, Judah

AU - Alkaade, Samer

AU - Amann, Stephen T.

AU - Anderson, Michelle A.

AU - Baillie, John

AU - Banks, Peter A.

AU - Brand, Randall E.

AU - Conwell, Darwin

AU - Coté, Gregory A.

AU - Forsmark, Christopher E.

AU - Gardner, Timothy B.

AU - Gelrud, Andres

AU - Guda, Nalini

AU - Lewis, Michele

AU - Money, Mary E.

AU - Muniraj, Thiruvengadam

AU - Sandhu, Bimaljit S.

AU - Sherman, Stuart

AU - Singh, Vikesh K.

AU - Slivka, Adam

AU - Tang, Gong

AU - Wilcox, C. Mel

AU - Whitcomb, David C.

AU - Yadav, Dhiraj

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Background: Multiple pathogenic genetic variants are associated with pancreatitis in patients of European (EA) and Asian ancestries, but studies on patients of African ancestry (AA) are lacking. We evaluated the prevalence of known genetic variations in African-American subjects in the US. Methods: We studied prospectively enrolled controls (n = 238) and patients with chronic (CP) (n = 232) or recurrent acute pancreatitis (RAP) (n = 45) in the NAPS2 studies from 2000-2014 of self-identified AA. Demographic and phenotypic information was obtained from structured questionnaires. Ancestry and admixture were evaluated by principal component analysis (PCA). Genotyping was performed for pathogenic genetic variants in PRSS1, SPINK1, CFTR and CTRC. Prevalence of disease-associated variants in NAPS2 subjects of AA and EA was compared. Results: When compared with CP subjects of EA (n = 862), prevalence of established pathogenic genetic variants was infrequent in AA patients with CP, overall (29 vs. 8.19%, OR 4.60, 95% CI 2.74–7.74, p < 0.001), and after stratification by alcohol etiology (p < 0.001). On PCA, AA cases were more heterogeneous but distinct from EA subjects; no difference was observed between AA subjects with and without CP-associated variants. Of 19 A A patients with CP who had pathogenic genetic variants, 2 had variants in PRSS1 (R122H, R122C), 4 in SPINK1 (all N34S heterozygotes), 12 in CFTR (2 CFTRsev, 9 CFTRBD, 1 compound heterozygote with CFTRsev and CFTRBD), and 1 in CTRC (R254W). Conclusion: Pathogenic genetic variants reported in EA patients are significantly less common in AA patients. Further studies are needed to determine the complex risk factors for AA subjects with pancreatitis.

AB - Background: Multiple pathogenic genetic variants are associated with pancreatitis in patients of European (EA) and Asian ancestries, but studies on patients of African ancestry (AA) are lacking. We evaluated the prevalence of known genetic variations in African-American subjects in the US. Methods: We studied prospectively enrolled controls (n = 238) and patients with chronic (CP) (n = 232) or recurrent acute pancreatitis (RAP) (n = 45) in the NAPS2 studies from 2000-2014 of self-identified AA. Demographic and phenotypic information was obtained from structured questionnaires. Ancestry and admixture were evaluated by principal component analysis (PCA). Genotyping was performed for pathogenic genetic variants in PRSS1, SPINK1, CFTR and CTRC. Prevalence of disease-associated variants in NAPS2 subjects of AA and EA was compared. Results: When compared with CP subjects of EA (n = 862), prevalence of established pathogenic genetic variants was infrequent in AA patients with CP, overall (29 vs. 8.19%, OR 4.60, 95% CI 2.74–7.74, p < 0.001), and after stratification by alcohol etiology (p < 0.001). On PCA, AA cases were more heterogeneous but distinct from EA subjects; no difference was observed between AA subjects with and without CP-associated variants. Of 19 A A patients with CP who had pathogenic genetic variants, 2 had variants in PRSS1 (R122H, R122C), 4 in SPINK1 (all N34S heterozygotes), 12 in CFTR (2 CFTRsev, 9 CFTRBD, 1 compound heterozygote with CFTRsev and CFTRBD), and 1 in CTRC (R254W). Conclusion: Pathogenic genetic variants reported in EA patients are significantly less common in AA patients. Further studies are needed to determine the complex risk factors for AA subjects with pancreatitis.

KW - African ancestry

KW - African-American

KW - Alcohol

KW - Blacks

KW - Genetics

KW - Pancreatitis

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