L [αS,5S] α amino 3 chloro 4,5 dihydro 5 isoxazoleacetic acid (NSC 163501), an antibiotic elaborated by Streptomyces sviceus, has been shown to be a powerful inhibitor of many mammalian and bacterial reactions involving the transfer of nitrogen from the γ carboxamide of L glutamine. Thus, the utilization of L glutamine for the synthesis of carbamyl phosphate, L asparagine, guanosine 5' monophosphate, cytidine 5' triphosphate, N formylglycinamidine ribonucleotide, NAD, glucosamine 6 phosphate, and anthranilic acid is strongly or totally inhibited by a concentration of NSC 163501 of 1 x 10-3 M. L Glutamate synthetase of Escherichia coli is only modestly inhibited and 5 phosphoribosylamine synthesis in fetal rat liver is comparatively refractory to inhibition. NSC 163501 treatment of L1210 cells growing in a low L glutamine culture medium produced arrest in G1 or early S phase. Of the amino acids tested, only L glutamine antagonized such growth inhibition.
|Original language||English (US)|
|Number of pages||11|
|State||Published - Jan 1 1975|
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