L-arginine supplementation protects exercise performance and structural integrity of muscle fibers after a single bout of eccentric exercise in rats

Yulia N. Lomonosova, Boris S. Shenkman, Grigorii R. Kalamkarov, Tatiana Kostrominova, Tatyana L. Nemirovskaya

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Eccentric exercise is known to disrupt sarcolemmal integrity and induce damage of skeletal muscle fibers. We hypothesized that L-arginine (L-Arg; nitric oxide synthase (NOS) substrate) supplementation prior to a single bout of eccentric exercise would diminish exercise-induced damage. In addition, we used N-nitro-L-arginine methyl ester hydrochloride (L-NAME; NOS inhibitor) to clarify the role of native NOS activity in the development of exercise-induced muscle damage. Rats were divided into four groups: non-treated control (C), downhill running with (RA) or without (R) L-Arg supplementation and downhill running with L-NAME supplementation (RN). Twenty four hours following eccentric exercise seven rats in each group were sacrificed and soleus muscles were dissected and frozen for further analysis. The remaining seven rats in each group were subjected to the exercise performance test. Our experiments showed that L-Arg supplementation prior to a single bout of eccentric exercise improved subsequent exercise performance capacity tests in RA rats when compared with R, RN and C rats by 37%, 27% and 13%, respectively. This outcome is mediated by L-Arg protection against post-exercise damage of sarcolemma (2.26-and 0.87-fold less than R and RN groups, respectively), reduced numbers of damaged muscle fibers indicated by the reduced loss of desmin content in the muscle (15% and 25% less than R and RN groups, respectively), and diminished μ-calpain mRNA up-regulation (42% and 30% less than R and RN groups, respectively). In conclusion, our study indicates that L-Arg supplementation prior to a single bout of eccentric exercise alleviates muscle fiber damage and preserves exercise performance capacity.

Original languageEnglish (US)
Article numbere94448
JournalPLoS One
Volume9
Issue number4
DOIs
StatePublished - Apr 15 2014

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Structural integrity
muscle fibers
arginine
Arginine
Muscle
Rats
exercise
Muscles
Fibers
Nitric Oxide Synthase
rats
NG-Nitroarginine Methyl Ester
Running
nitric oxide synthase
Sarcolemma
Calpain
Desmin
Skeletal Muscle Fibers
Exercise Test
muscles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

L-arginine supplementation protects exercise performance and structural integrity of muscle fibers after a single bout of eccentric exercise in rats. / Lomonosova, Yulia N.; Shenkman, Boris S.; Kalamkarov, Grigorii R.; Kostrominova, Tatiana; Nemirovskaya, Tatyana L.

In: PLoS One, Vol. 9, No. 4, e94448, 15.04.2014.

Research output: Contribution to journalArticle

Lomonosova, Yulia N. ; Shenkman, Boris S. ; Kalamkarov, Grigorii R. ; Kostrominova, Tatiana ; Nemirovskaya, Tatyana L. / L-arginine supplementation protects exercise performance and structural integrity of muscle fibers after a single bout of eccentric exercise in rats. In: PLoS One. 2014 ; Vol. 9, No. 4.
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