l-asparaginases from Mycobacterium tuberculosis strains H37Rv and H37Ra

H. N. Jayaram, T. Ramakrishnan, C. S. Vaidyanathan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

M. tuberculosis H37Ra possesses two l-asparaginases while the H37Rv strain possesses only a single enzyme. These enzymes have been purified and their properties studied. The two l-asparaginases in H37Ra strain differ from each other in pH optima, heat inactivation, Michaelis constant and effects of inhibitors, while one of them resembles the single l-asparaginase present in the H37Rv strain. l-Cysteine inhibits both l-asparaginases in an allosteric manner probably because it is one of the end-products in l-asparagine metabolism. This is the first time that a qualitative difference has been reported in the enzyme pattern between the avirulent and virulent strains of M. tuberculosis.

Original languageEnglish (US)
Pages (from-to)165-174
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume126
Issue number1
StatePublished - Jul 1968
Externally publishedYes

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Asparaginase
Mycobacterium tuberculosis
Enzymes
Asparagine
Metabolism
Cysteine
Hot Temperature

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Jayaram, H. N., Ramakrishnan, T., & Vaidyanathan, C. S. (1968). l-asparaginases from Mycobacterium tuberculosis strains H37Rv and H37Ra. Archives of Biochemistry and Biophysics, 126(1), 165-174.

l-asparaginases from Mycobacterium tuberculosis strains H37Rv and H37Ra. / Jayaram, H. N.; Ramakrishnan, T.; Vaidyanathan, C. S.

In: Archives of Biochemistry and Biophysics, Vol. 126, No. 1, 07.1968, p. 165-174.

Research output: Contribution to journalArticle

Jayaram, HN, Ramakrishnan, T & Vaidyanathan, CS 1968, 'l-asparaginases from Mycobacterium tuberculosis strains H37Rv and H37Ra', Archives of Biochemistry and Biophysics, vol. 126, no. 1, pp. 165-174.
Jayaram, H. N. ; Ramakrishnan, T. ; Vaidyanathan, C. S. / l-asparaginases from Mycobacterium tuberculosis strains H37Rv and H37Ra. In: Archives of Biochemistry and Biophysics. 1968 ; Vol. 126, No. 1. pp. 165-174.
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