l-asparaginases from Mycobacterium tuberculosis strains H37Rv and H37Ra

H. N. Jayaram, T. Ramakrishnan, C. S. Vaidyanathan

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

M. tuberculosis H37Ra possesses two l-asparaginases while the H37Rv strain possesses only a single enzyme. These enzymes have been purified and their properties studied. The two l-asparaginases in H37Ra strain differ from each other in pH optima, heat inactivation, Michaelis constant and effects of inhibitors, while one of them resembles the single l-asparaginase present in the H37Rv strain. l-Cysteine inhibits both l-asparaginases in an allosteric manner probably because it is one of the end-products in l-asparagine metabolism. This is the first time that a qualitative difference has been reported in the enzyme pattern between the avirulent and virulent strains of M. tuberculosis.

Original languageEnglish (US)
Pages (from-to)165-174
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume126
Issue number1
DOIs
StatePublished - Jul 1968

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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