L-carnitine protects human retinal pigment epithelial cells from oxidative damage

Farrukh A. Shamsi, Imtiaz A. Chaudhry, Mike E. Boulton, Ali A. Al-Rajhi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Purpose: To determine the efficacy of L-carnitine (LC) against oxidative changes in human retinal pigment epithelium (RPE) cells. Methods: The RPE cells from human donor eyes were cultured in Hams F-10 medium. The effect of LC on H2O2-induced morphologic changes in the RPE cells was analyzed by light microscopy. Reduction in cell death after the impact of LC treatment on H2O2-treated cells was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assays. In addition, the effect of H2O2 on the activity of RPE-antioxidant enzymes, glutathione (GSH) and superoxide dismutase (SOD), and LC-induced protection was also determined. Results: LC protected the RPE cells by inhibiting the peroxide-induced cytopathic effect from 50% to 10%. Nuclear condensation observed in 40% of the H2O2-treated cells decreased to 20% after LC treatment. The MTT assays demonstrated that 100 μM oxidant caused appreciable cell death, which was reduced by LC treatment; however, 100% protection was not achieved. Significant peroxide-induced cell death was seen within 5 hr of H2O2 treatment, and a quantifiable reduction was observed after LC treatment for a similar time period. The change in the antioxidant potential of the RPE induced by oxidative stress was restored by LC treatment, as demonstrated by an increase in GSH and SOD activities. Conclusions: LC is capable of protecting the RPE cells from H2O2-induced oxidative damage, implying that micronutrients can have a positive effect and can play an important role in the treatment of oxidation-induced ocular disorders. Further studies are needed to understand the mechanism of LC-induced protection to the RPE cells.

Original languageEnglish (US)
Pages (from-to)575-584
Number of pages10
JournalCurrent Eye Research
Volume32
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

Retinal Pigments
Carnitine
Retinal Pigment Epithelium
Epithelial Cells
Cell Death
Peroxides
Superoxide Dismutase
Antioxidants
Micronutrients
Oxidants
Glutathione
Microscopy
Oxidative Stress

Keywords

  • Aging
  • Enzymes
  • L-carnitine
  • Morphology
  • Oxidation
  • RPE

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

L-carnitine protects human retinal pigment epithelial cells from oxidative damage. / Shamsi, Farrukh A.; Chaudhry, Imtiaz A.; Boulton, Mike E.; Al-Rajhi, Ali A.

In: Current Eye Research, Vol. 32, No. 6, 06.2007, p. 575-584.

Research output: Contribution to journalArticle

Shamsi, Farrukh A. ; Chaudhry, Imtiaz A. ; Boulton, Mike E. ; Al-Rajhi, Ali A. / L-carnitine protects human retinal pigment epithelial cells from oxidative damage. In: Current Eye Research. 2007 ; Vol. 32, No. 6. pp. 575-584.
@article{7bdabfc208bd44e996c407396bb7d5c9,
title = "L-carnitine protects human retinal pigment epithelial cells from oxidative damage",
abstract = "Purpose: To determine the efficacy of L-carnitine (LC) against oxidative changes in human retinal pigment epithelium (RPE) cells. Methods: The RPE cells from human donor eyes were cultured in Hams F-10 medium. The effect of LC on H2O2-induced morphologic changes in the RPE cells was analyzed by light microscopy. Reduction in cell death after the impact of LC treatment on H2O2-treated cells was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assays. In addition, the effect of H2O2 on the activity of RPE-antioxidant enzymes, glutathione (GSH) and superoxide dismutase (SOD), and LC-induced protection was also determined. Results: LC protected the RPE cells by inhibiting the peroxide-induced cytopathic effect from 50{\%} to 10{\%}. Nuclear condensation observed in 40{\%} of the H2O2-treated cells decreased to 20{\%} after LC treatment. The MTT assays demonstrated that 100 μM oxidant caused appreciable cell death, which was reduced by LC treatment; however, 100{\%} protection was not achieved. Significant peroxide-induced cell death was seen within 5 hr of H2O2 treatment, and a quantifiable reduction was observed after LC treatment for a similar time period. The change in the antioxidant potential of the RPE induced by oxidative stress was restored by LC treatment, as demonstrated by an increase in GSH and SOD activities. Conclusions: LC is capable of protecting the RPE cells from H2O2-induced oxidative damage, implying that micronutrients can have a positive effect and can play an important role in the treatment of oxidation-induced ocular disorders. Further studies are needed to understand the mechanism of LC-induced protection to the RPE cells.",
keywords = "Aging, Enzymes, L-carnitine, Morphology, Oxidation, RPE",
author = "Shamsi, {Farrukh A.} and Chaudhry, {Imtiaz A.} and Boulton, {Mike E.} and Al-Rajhi, {Ali A.}",
year = "2007",
month = "6",
doi = "10.1080/02713680701363833",
language = "English (US)",
volume = "32",
pages = "575--584",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "6",

}

TY - JOUR

T1 - L-carnitine protects human retinal pigment epithelial cells from oxidative damage

AU - Shamsi, Farrukh A.

AU - Chaudhry, Imtiaz A.

AU - Boulton, Mike E.

AU - Al-Rajhi, Ali A.

PY - 2007/6

Y1 - 2007/6

N2 - Purpose: To determine the efficacy of L-carnitine (LC) against oxidative changes in human retinal pigment epithelium (RPE) cells. Methods: The RPE cells from human donor eyes were cultured in Hams F-10 medium. The effect of LC on H2O2-induced morphologic changes in the RPE cells was analyzed by light microscopy. Reduction in cell death after the impact of LC treatment on H2O2-treated cells was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assays. In addition, the effect of H2O2 on the activity of RPE-antioxidant enzymes, glutathione (GSH) and superoxide dismutase (SOD), and LC-induced protection was also determined. Results: LC protected the RPE cells by inhibiting the peroxide-induced cytopathic effect from 50% to 10%. Nuclear condensation observed in 40% of the H2O2-treated cells decreased to 20% after LC treatment. The MTT assays demonstrated that 100 μM oxidant caused appreciable cell death, which was reduced by LC treatment; however, 100% protection was not achieved. Significant peroxide-induced cell death was seen within 5 hr of H2O2 treatment, and a quantifiable reduction was observed after LC treatment for a similar time period. The change in the antioxidant potential of the RPE induced by oxidative stress was restored by LC treatment, as demonstrated by an increase in GSH and SOD activities. Conclusions: LC is capable of protecting the RPE cells from H2O2-induced oxidative damage, implying that micronutrients can have a positive effect and can play an important role in the treatment of oxidation-induced ocular disorders. Further studies are needed to understand the mechanism of LC-induced protection to the RPE cells.

AB - Purpose: To determine the efficacy of L-carnitine (LC) against oxidative changes in human retinal pigment epithelium (RPE) cells. Methods: The RPE cells from human donor eyes were cultured in Hams F-10 medium. The effect of LC on H2O2-induced morphologic changes in the RPE cells was analyzed by light microscopy. Reduction in cell death after the impact of LC treatment on H2O2-treated cells was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] assays. In addition, the effect of H2O2 on the activity of RPE-antioxidant enzymes, glutathione (GSH) and superoxide dismutase (SOD), and LC-induced protection was also determined. Results: LC protected the RPE cells by inhibiting the peroxide-induced cytopathic effect from 50% to 10%. Nuclear condensation observed in 40% of the H2O2-treated cells decreased to 20% after LC treatment. The MTT assays demonstrated that 100 μM oxidant caused appreciable cell death, which was reduced by LC treatment; however, 100% protection was not achieved. Significant peroxide-induced cell death was seen within 5 hr of H2O2 treatment, and a quantifiable reduction was observed after LC treatment for a similar time period. The change in the antioxidant potential of the RPE induced by oxidative stress was restored by LC treatment, as demonstrated by an increase in GSH and SOD activities. Conclusions: LC is capable of protecting the RPE cells from H2O2-induced oxidative damage, implying that micronutrients can have a positive effect and can play an important role in the treatment of oxidation-induced ocular disorders. Further studies are needed to understand the mechanism of LC-induced protection to the RPE cells.

KW - Aging

KW - Enzymes

KW - L-carnitine

KW - Morphology

KW - Oxidation

KW - RPE

UR - http://www.scopus.com/inward/record.url?scp=34447120697&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447120697&partnerID=8YFLogxK

U2 - 10.1080/02713680701363833

DO - 10.1080/02713680701363833

M3 - Article

VL - 32

SP - 575

EP - 584

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 6

ER -