L-type calcium channels mediate mechanically induced bone formation in vivo

Jiliang Li, Randall L. Duncan, David Burr, Charles H. Turner

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Cell and tissue culture studies suggest that the long-lasting (L-type) voltage-sensitive calcium channels (VSCC) play a role in the signaling cascade in bone cells after mechanical loading. We investigated whether the L-type VSCC mediates mechanically induced bone formation in vivo using two L-type VSCC antagonists verapamil and nifedipine. Female Sprague-Dawley rats were divided into five groups: control group (Veh), two verapamil-treated groups (20 mg/kg, Vera-L; 100 mg/kg, Vera-H), and two nifedipine-treated groups (20 mg/kg, Nife-L; 100 mg/kg, Nife-H). One bout of mechanical loading was applied to the right tibia 90 minutes after oral administration of verapamil or 30 minutes after oral administration of nifedipine. Mechanical loading increased mineralizing surface (MS/bone surface [BS]), mineral apposition rate (MAR), and bone formation rate (BFR/BS) on the endocortical surface in loaded tibias of control animals compared with nonloaded (left) tibias. Verapamil and nifedipine suppressed the load-induced increase in BFR/BS observed in vehicle-treated controls by 56-61% (p < 0.01) and 56-74% (p < 0.01), respectively. Yet, significant differences in MS/BS and BFR/BS between right and left limbs were found in verapamil- and nifedipine-treated animals, indicating that the treatments did not completely abolish load-induced bone formation. This study shows that blocking the L-type calcium channel in vivo substantially suppresses the mechanically induced increase in bone formation that normally would occur and suggests that the L-type calcium channel mediates mechanically induced bone adaptation in vivo.

Original languageEnglish
Pages (from-to)1795-1800
Number of pages6
JournalJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume17
Issue number10
StatePublished - Oct 1 2002

Fingerprint

L-Type Calcium Channels
Osteogenesis
Nifedipine
Verapamil
Bone and Bones
Tibia
Calcium Channels
Oral Administration
Calcium Channel Blockers
Minerals
Sprague Dawley Rats
Extremities
Cell Culture Techniques
Control Groups

Keywords

  • Bone modeling
  • Calcium channel
  • Histomorphometry
  • Mechanical loading
  • Rat tibia

ASJC Scopus subject areas

  • Surgery

Cite this

L-type calcium channels mediate mechanically induced bone formation in vivo. / Li, Jiliang; Duncan, Randall L.; Burr, David; Turner, Charles H.

In: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 17, No. 10, 01.10.2002, p. 1795-1800.

Research output: Contribution to journalArticle

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