Label-free bond-selective imaging by listening to vibrationally excited molecules

Han Wei Wang, Ning Chai, Pu Wang, Song Hu, Wei Dou, David Umulis, Lihong V. Wang, Michael Sturek, Robert Lucht, Ji Xin Cheng

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

We report the realization of vibrational photoacoustic (VPA) microscopy using optical excitation of molecular overtone vibration and acoustic detection of the resultant pressure transients. Our approach eliminates the tissue scattering problem encountered in near-infrared spectroscopy and enables depth-resolved signal collection. The 2nd overtone of the CH bond stretch around 8300cm-1, where blood interference is minimal, is excited. We demonstrate 3D VPA imaging of lipid-rich atherosclerotic plaques by excitation from the artery lumen, and lipid storage in live Drosophila larvae, with millimeter-scale penetration depth.

Original languageEnglish
Article number238106
JournalPhysical Review Letters
Volume106
Issue number23
DOIs
StatePublished - Jun 10 2011

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lipids
photoacoustic microscopy
sound detecting and ranging
transient pressures
larvae
Drosophila
harmonics
lumens
arteries
excitation
blood
molecules
penetration
infrared spectroscopy
methylidyne
interference
vibration
scattering

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Wang, H. W., Chai, N., Wang, P., Hu, S., Dou, W., Umulis, D., ... Cheng, J. X. (2011). Label-free bond-selective imaging by listening to vibrationally excited molecules. Physical Review Letters, 106(23), [238106]. https://doi.org/10.1103/PhysRevLett.106.238106

Label-free bond-selective imaging by listening to vibrationally excited molecules. / Wang, Han Wei; Chai, Ning; Wang, Pu; Hu, Song; Dou, Wei; Umulis, David; Wang, Lihong V.; Sturek, Michael; Lucht, Robert; Cheng, Ji Xin.

In: Physical Review Letters, Vol. 106, No. 23, 238106, 10.06.2011.

Research output: Contribution to journalArticle

Wang, HW, Chai, N, Wang, P, Hu, S, Dou, W, Umulis, D, Wang, LV, Sturek, M, Lucht, R & Cheng, JX 2011, 'Label-free bond-selective imaging by listening to vibrationally excited molecules', Physical Review Letters, vol. 106, no. 23, 238106. https://doi.org/10.1103/PhysRevLett.106.238106
Wang, Han Wei ; Chai, Ning ; Wang, Pu ; Hu, Song ; Dou, Wei ; Umulis, David ; Wang, Lihong V. ; Sturek, Michael ; Lucht, Robert ; Cheng, Ji Xin. / Label-free bond-selective imaging by listening to vibrationally excited molecules. In: Physical Review Letters. 2011 ; Vol. 106, No. 23.
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