Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy

Thuc T. Le, Ingeborg M. Langohr, Matthew J. Locker, Michael Sturek, Ji Xin Cheng

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Arterial tissues collected from Ossabaw swine bearing metabolic syndrome-induced cardiovascular plaques are characterized by multimodal nonlinear optical microscopy that allows coherent anti-Stokes Raman scattering, second-harmonic generation, and two-photon excitation fluorescence imaging on the same platform. Significant components of arterial walls and atherosclerotic lesions, including endothelial cells, extracellular lipid droplets, lipid-rich cells, low-density lipoprotein aggregates, collagen, and elastin are imaged without any labeling. Emission spectra of these components are obtained by nonlinear optical microspectrometry. The nonlinear optical contrast is compared with histology of the same sample. Multimodal nonlinear optical imaging of plaque composition also allows identification of atherosclerotic regions that are vulnerable to rupture risk. The demonstrated capability of nonlinear optical microscopy for label-free molecular imaging of atherosclerotic lesions with 3-D submicrometric resolution suggests its potential application to the diagnosis of atherosclerotic plaques, determination of their rupture risk, and design of individualized drug therapy based on plaque composition.

Original languageEnglish
Article number054007
JournalJournal of Biomedical Optics
Volume12
Issue number5
DOIs
StatePublished - 2007

Fingerprint

Molecular imaging
Molecular Imaging
Optical Imaging
lesions
Lipids
Optical microscopy
lipids
Labels
Rupture
Microscopy
Bearings (structural)
Metabolic Syndrome X
elastin
microscopy
lipoproteins
Elastin
Drug therapy
Imaging techniques
Lipoproteins
Histology

Keywords

  • Atherosclerosis
  • Coherent anti-Stokes Raman scattering
  • Nonlinear optics
  • Obesity
  • Plaque
  • Second-harmonic generation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials

Cite this

Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy. / Le, Thuc T.; Langohr, Ingeborg M.; Locker, Matthew J.; Sturek, Michael; Cheng, Ji Xin.

In: Journal of Biomedical Optics, Vol. 12, No. 5, 054007, 2007.

Research output: Contribution to journalArticle

Le, Thuc T. ; Langohr, Ingeborg M. ; Locker, Matthew J. ; Sturek, Michael ; Cheng, Ji Xin. / Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy. In: Journal of Biomedical Optics. 2007 ; Vol. 12, No. 5.
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