Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy

Ping Wang, Junjie Li, Pu Wang, Chun Rui Hu, Delong Zhang, Michael Sturek, Ji Xin Cheng

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.

Original languageEnglish (US)
Pages (from-to)13042-13046
Number of pages5
JournalAngewandte Chemie - International Edition
Volume52
Issue number49
DOIs
StatePublished - Dec 2 2013

Fingerprint

Stimulated Raman scattering
Cholesterol
Labels
Microscopic examination
Tissue
Imaging techniques
Biomolecules
Assays

Keywords

  • cholesterol
  • lipids
  • stimulated Raman scattering
  • tissue analysis
  • vibrational spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy. / Wang, Ping; Li, Junjie; Wang, Pu; Hu, Chun Rui; Zhang, Delong; Sturek, Michael; Cheng, Ji Xin.

In: Angewandte Chemie - International Edition, Vol. 52, No. 49, 02.12.2013, p. 13042-13046.

Research output: Contribution to journalArticle

Wang, Ping ; Li, Junjie ; Wang, Pu ; Hu, Chun Rui ; Zhang, Delong ; Sturek, Michael ; Cheng, Ji Xin. / Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy. In: Angewandte Chemie - International Edition. 2013 ; Vol. 52, No. 49. pp. 13042-13046.
@article{c3832da6ecc243d0a3ff5d5fa16cb64f,
title = "Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy",
abstract = "A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.",
keywords = "cholesterol, lipids, stimulated Raman scattering, tissue analysis, vibrational spectroscopy",
author = "Ping Wang and Junjie Li and Pu Wang and Hu, {Chun Rui} and Delong Zhang and Michael Sturek and Cheng, {Ji Xin}",
year = "2013",
month = "12",
day = "2",
doi = "10.1002/anie.201306234",
language = "English (US)",
volume = "52",
pages = "13042--13046",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "49",

}

TY - JOUR

T1 - Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy

AU - Wang, Ping

AU - Li, Junjie

AU - Wang, Pu

AU - Hu, Chun Rui

AU - Zhang, Delong

AU - Sturek, Michael

AU - Cheng, Ji Xin

PY - 2013/12/2

Y1 - 2013/12/2

N2 - A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.

AB - A finger on the pulse: Current molecular analysis of cells and tissues routinely relies on separation, enrichment, and subsequent measurements by various assays. Now, a platform of hyperspectral stimulated Raman scattering microscopy has been developed for the fast, quantitative, and label-free imaging of biomolecules in intact tissues using spectroscopic fingerprints as the contrast mechanism.

KW - cholesterol

KW - lipids

KW - stimulated Raman scattering

KW - tissue analysis

KW - vibrational spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=84888599621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888599621&partnerID=8YFLogxK

U2 - 10.1002/anie.201306234

DO - 10.1002/anie.201306234

M3 - Article

C2 - 24127161

AN - SCOPUS:84888599621

VL - 52

SP - 13042

EP - 13046

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 49

ER -