Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential

Hyeon Jeong Lee, Delong Zhang, Ying Jiang, Xiangbing Wu, Pei Yu Shih, Chien Sheng Liao, Brittani Bungart, Xiao-Ming Xu, Ryan Drenan, Edward Bartlett, Ji Xin Cheng

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Detecting membrane potentials is critical for understanding how neuronal networks process information. We report a vibrational spectroscopic signature of neuronal membrane potentials identified through hyperspectral stimulated Raman scattering (SRS) imaging of patched primary neurons. High-speed SRS imaging allowed direct visualization of puff-induced depolarization of multiple neurons in mouse brain slices, confirmed by simultaneous calcium imaging. The observed signature, partially dependent on sodium ion influx, is interpreted as ion interactions on the CH3 Fermi resonance peak in proteins. By implementing a dual-SRS balanced detection scheme, we detected single action potentials in electrically stimulated neurons. These results collectively demonstrate the potential of sensing neuronal activities at multiple sites with a label-free vibrational microscope.

Original languageEnglish (US)
Pages (from-to)1932-1936
Number of pages5
JournalJournal of Physical Chemistry Letters
Volume8
Issue number9
DOIs
StatePublished - May 4 2017

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Stimulated Raman scattering
Neurons
Labels
neurons
membranes
Membranes
Imaging techniques
Raman spectra
Ions
Depolarization
signatures
Calcium
Brain
Microscopes
Visualization
Sodium
depolarization
brain
Proteins
mice

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Lee, H. J., Zhang, D., Jiang, Y., Wu, X., Shih, P. Y., Liao, C. S., ... Cheng, J. X. (2017). Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential. Journal of Physical Chemistry Letters, 8(9), 1932-1936. https://doi.org/10.1021/acs.jpclett.7b00575

Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential. / Lee, Hyeon Jeong; Zhang, Delong; Jiang, Ying; Wu, Xiangbing; Shih, Pei Yu; Liao, Chien Sheng; Bungart, Brittani; Xu, Xiao-Ming; Drenan, Ryan; Bartlett, Edward; Cheng, Ji Xin.

In: Journal of Physical Chemistry Letters, Vol. 8, No. 9, 04.05.2017, p. 1932-1936.

Research output: Contribution to journalArticle

Lee, HJ, Zhang, D, Jiang, Y, Wu, X, Shih, PY, Liao, CS, Bungart, B, Xu, X-M, Drenan, R, Bartlett, E & Cheng, JX 2017, 'Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential', Journal of Physical Chemistry Letters, vol. 8, no. 9, pp. 1932-1936. https://doi.org/10.1021/acs.jpclett.7b00575
Lee, Hyeon Jeong ; Zhang, Delong ; Jiang, Ying ; Wu, Xiangbing ; Shih, Pei Yu ; Liao, Chien Sheng ; Bungart, Brittani ; Xu, Xiao-Ming ; Drenan, Ryan ; Bartlett, Edward ; Cheng, Ji Xin. / Label-Free Vibrational Spectroscopic Imaging of Neuronal Membrane Potential. In: Journal of Physical Chemistry Letters. 2017 ; Vol. 8, No. 9. pp. 1932-1936.
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