Laboratory assays in pediatric endocrinology: Common aspects

Jon Nakamoto, John S. Fuqua

Research output: Contribution to journalReview article

7 Scopus citations

Abstract

With a rapidly expanding armamentarium of tests, endocrinologists need the ability to critically assess a laboratory assay and to understand its application. There are numerous important issues to consider. Analytical validation ensures that an assay meets standards for sensitivity, specificity and reproducibility. Clinical validation allows the results of a test to be effectively applied to a given clinical situation. Antibody-based endocrine assays are typically either competitive or non-competitive. For competitive assays, an interference that decreases signal wilt lead to an apparent increase in the analyte concentration, while an interference that decreases signal in a non-competitive assay will cause an apparent decrease in the analyte concentration. Mass spectrometry is rapidly increasing in use and has its own promises and pitfalls. Numerous methods exist for determining reference intervals, each giving different results. Making endocrine diagnoses based on such intervals may be overly simplistic and collaboration between clinicians and the laboratory to establish clinical thresholds would be far more informative.

Original languageEnglish (US)
Pages (from-to)539-554
Number of pages16
JournalPediatric Endocrinology Reviews
Volume5
Issue numberSUPPL. 1
StatePublished - Oct 1 2007

Keywords

  • Analytic validation
  • Assay interference
  • Clinical validation
  • Immunometric assay
  • Mass spectrometry
  • Radioimmunoassay
  • Reference interval

ASJC Scopus subject areas

  • Plant Science
  • Aquatic Science
  • Ecology

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