Laboratory assays in pediatric endocrinology

Common aspects

Jon Nakamoto, John Fuqua

Research output: Contribution to journalArticle

Abstract

With a rapidly expanding armamentarium of tests, endocrinologists need the ability to critically assess a laboratory assay and to understand its application. There are numerous important issues to consider. Analytical validation ensures that an assay meets standards for sensitivity, specificity and reproducibility. Clinical validation allows the results of a test to be effectively applied to a given clinical situation. Antibody-based endocrine assays are typically either competitive or non-competitive. For competitive assays, an interference that decreases signal wilt lead to an apparent increase in the analyte concentration, while an interference that decreases signal in a non-competitive assay will cause an apparent decrease in the analyte concentration. Mass spectrometry is rapidly increasing in use and has its own promises and pitfalls. Numerous methods exist for determining reference intervals, each giving different results. Making endocrine diagnoses based on such intervals may be overly simplistic and collaboration between clinicians and the laboratory to establish clinical thresholds would be far more informative.

Original languageEnglish
Pages (from-to)539-554
Number of pages16
JournalArab Gulf Journal of Scientific Research
Volume25
Issue number1-2
StatePublished - Mar 2007

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endocrinology
assay
assays
wilt
reproducibility
antibody
mass spectrometry
testing
laboratory
antibodies

Keywords

  • Analytic validation
  • Assay interference
  • Clinical validation
  • Immunometric assay
  • Mass spectrometry
  • Radioimmunoassay
  • Reference interval

ASJC Scopus subject areas

  • Plant Science
  • Aquatic Science
  • Ecology

Cite this

Laboratory assays in pediatric endocrinology : Common aspects. / Nakamoto, Jon; Fuqua, John.

In: Arab Gulf Journal of Scientific Research, Vol. 25, No. 1-2, 03.2007, p. 539-554.

Research output: Contribution to journalArticle

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