Laboratory assessment of the anticaries potential of a new dentifrice

Frank Lippert, Evelyn E. Newby, R. J M Lynch, Vijeash K. Chauhan, Bruce R. Schemehorn

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: The aim of the present study was to investigate the anticaries potential of a new sodium fluoride dentifrice in comparison to two commercial dentifrices containing different fluoride compounds by determining enamel fluoride uptake (EFU) and early caries lesion remineralization (REM) in an established in vitro caries remineralization/demineralization pH cycling model. Methods: Test products were: new dentifrice formulation in a fluoride dose-response (0; 675; 1426 ppm F as sodium fluoride [NaF-0; NaF-675; NaF]); Elmex® Kariesschutz (1400 ppm F as amine fluoride [AmFJ); and Oral-B® Pro-Expert (1450 ppm F-1100 ppm F as stannous fluoride and 350 ppm F as sodium fluoride [SnNaF]). Artificial caries-like lesions were formed in human enamel specimens by immersion in lactic acid buffer (LA). Specimens were then subjected to a daily cycling regime for 20 days comprising four one-minute dentifrice slurry treatments (prepared in pooled human saliva), and one four-hour LA challenge and remineralization in pooled human saliva. After 20 days, REM was evaluated as the change in surface Vickers microhardness from lesion baseline and EFU using the microdrill technique. The data were analyzed using ANOVA. Results: A good fluoride dose-response was established for EFU and REM, with NaF delivering greater EFU and REM than NaF-675, which was superior to NaF-0 (p <0.05). The new dentifrice NaF also showed greater EFU and REM than AmF and SnNaF (p <0.05). In EFU, AmF and SnNaF were as efficacious as NaF-675 and superior to NaF-0 (p <0.05). AmF and NaF-675 were also comparable in REM, whereas both products exhibited superior REM vs. SnNaF (p <0.05), which was superior to NaF-0 (p <0.05). Conclusion: The present study has demonstrated that fluoride dentifrices vary in their capability of enhancing anticaries potential as determined using an established in vitro caries cycling model. The new dentifrice NaF showed superior predicted anticaries potential compared to the two commercial dentifrices AmF and SnNaF in this model, which demonstrates the importance of fluoride compound and formulation excipients on driving anticaries potential in vitro.

Original languageEnglish (US)
Pages (from-to)45-49
Number of pages5
JournalJournal of Clinical Dentistry
Volume20
Issue number2
StatePublished - 2009
Externally publishedYes

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Dentifrices
Fluorides
Dental Enamel
Sodium Fluoride
Saliva
Lactic Acid
Buffers
Tin Fluorides
Excipients
Immersion
Amines
Analysis of Variance

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Lippert, F., Newby, E. E., Lynch, R. J. M., Chauhan, V. K., & Schemehorn, B. R. (2009). Laboratory assessment of the anticaries potential of a new dentifrice. Journal of Clinical Dentistry, 20(2), 45-49.

Laboratory assessment of the anticaries potential of a new dentifrice. / Lippert, Frank; Newby, Evelyn E.; Lynch, R. J M; Chauhan, Vijeash K.; Schemehorn, Bruce R.

In: Journal of Clinical Dentistry, Vol. 20, No. 2, 2009, p. 45-49.

Research output: Contribution to journalArticle

Lippert, F, Newby, EE, Lynch, RJM, Chauhan, VK & Schemehorn, BR 2009, 'Laboratory assessment of the anticaries potential of a new dentifrice', Journal of Clinical Dentistry, vol. 20, no. 2, pp. 45-49.
Lippert, Frank ; Newby, Evelyn E. ; Lynch, R. J M ; Chauhan, Vijeash K. ; Schemehorn, Bruce R. / Laboratory assessment of the anticaries potential of a new dentifrice. In: Journal of Clinical Dentistry. 2009 ; Vol. 20, No. 2. pp. 45-49.
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abstract = "Objective: The aim of the present study was to investigate the anticaries potential of a new sodium fluoride dentifrice in comparison to two commercial dentifrices containing different fluoride compounds by determining enamel fluoride uptake (EFU) and early caries lesion remineralization (REM) in an established in vitro caries remineralization/demineralization pH cycling model. Methods: Test products were: new dentifrice formulation in a fluoride dose-response (0; 675; 1426 ppm F as sodium fluoride [NaF-0; NaF-675; NaF]); Elmex{\circledR} Kariesschutz (1400 ppm F as amine fluoride [AmFJ); and Oral-B{\circledR} Pro-Expert (1450 ppm F-1100 ppm F as stannous fluoride and 350 ppm F as sodium fluoride [SnNaF]). Artificial caries-like lesions were formed in human enamel specimens by immersion in lactic acid buffer (LA). Specimens were then subjected to a daily cycling regime for 20 days comprising four one-minute dentifrice slurry treatments (prepared in pooled human saliva), and one four-hour LA challenge and remineralization in pooled human saliva. After 20 days, REM was evaluated as the change in surface Vickers microhardness from lesion baseline and EFU using the microdrill technique. The data were analyzed using ANOVA. Results: A good fluoride dose-response was established for EFU and REM, with NaF delivering greater EFU and REM than NaF-675, which was superior to NaF-0 (p <0.05). The new dentifrice NaF also showed greater EFU and REM than AmF and SnNaF (p <0.05). In EFU, AmF and SnNaF were as efficacious as NaF-675 and superior to NaF-0 (p <0.05). AmF and NaF-675 were also comparable in REM, whereas both products exhibited superior REM vs. SnNaF (p <0.05), which was superior to NaF-0 (p <0.05). Conclusion: The present study has demonstrated that fluoride dentifrices vary in their capability of enhancing anticaries potential as determined using an established in vitro caries cycling model. The new dentifrice NaF showed superior predicted anticaries potential compared to the two commercial dentifrices AmF and SnNaF in this model, which demonstrates the importance of fluoride compound and formulation excipients on driving anticaries potential in vitro.",
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AU - Lynch, R. J M

AU - Chauhan, Vijeash K.

AU - Schemehorn, Bruce R.

PY - 2009

Y1 - 2009

N2 - Objective: The aim of the present study was to investigate the anticaries potential of a new sodium fluoride dentifrice in comparison to two commercial dentifrices containing different fluoride compounds by determining enamel fluoride uptake (EFU) and early caries lesion remineralization (REM) in an established in vitro caries remineralization/demineralization pH cycling model. Methods: Test products were: new dentifrice formulation in a fluoride dose-response (0; 675; 1426 ppm F as sodium fluoride [NaF-0; NaF-675; NaF]); Elmex® Kariesschutz (1400 ppm F as amine fluoride [AmFJ); and Oral-B® Pro-Expert (1450 ppm F-1100 ppm F as stannous fluoride and 350 ppm F as sodium fluoride [SnNaF]). Artificial caries-like lesions were formed in human enamel specimens by immersion in lactic acid buffer (LA). Specimens were then subjected to a daily cycling regime for 20 days comprising four one-minute dentifrice slurry treatments (prepared in pooled human saliva), and one four-hour LA challenge and remineralization in pooled human saliva. After 20 days, REM was evaluated as the change in surface Vickers microhardness from lesion baseline and EFU using the microdrill technique. The data were analyzed using ANOVA. Results: A good fluoride dose-response was established for EFU and REM, with NaF delivering greater EFU and REM than NaF-675, which was superior to NaF-0 (p <0.05). The new dentifrice NaF also showed greater EFU and REM than AmF and SnNaF (p <0.05). In EFU, AmF and SnNaF were as efficacious as NaF-675 and superior to NaF-0 (p <0.05). AmF and NaF-675 were also comparable in REM, whereas both products exhibited superior REM vs. SnNaF (p <0.05), which was superior to NaF-0 (p <0.05). Conclusion: The present study has demonstrated that fluoride dentifrices vary in their capability of enhancing anticaries potential as determined using an established in vitro caries cycling model. The new dentifrice NaF showed superior predicted anticaries potential compared to the two commercial dentifrices AmF and SnNaF in this model, which demonstrates the importance of fluoride compound and formulation excipients on driving anticaries potential in vitro.

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