Lamin B is a prompt heat shock protein

Joseph Dynlacht, Michael D. Story, Wei Guo Zhu, Jeffrey Danner

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The cellular response to hyperthermia involves the increased synthesis of heat shock proteins (HSPs) within several hours after treatment. In addition, a subset of proteins has been shown to be increased immediately after heating. These 'prompt' HSPs are predominantly found in the nuclear matrix-intermediate filament fraction and are not present or detectable in unheated cells. Since the nuclear matrix has been suggested to be a target for heat-induced cell killing, prompt HSPs may play a prominent role in the heat shock response. Using Western blotting and flow cytometry, we found that an increase in the synthesis of lamin B, one of the major proteins of the nuclear lamina, is induced during heating at 45.5°C but not during heating at 42°C. Since it is an abundant protein which is constitutively expressed in mammalian cells, lamin B appears to be a unique member of the prompt HSP family. The kinetics of induction of lamin B during 45.5°C heating did not correlate with the dose-dependent reduction in cell survival. While increased levels of lamin B during 45.5°C heating do not appear to confer a survival advantage directly, a possible role for lamin B in cellular recovery after heat shock cannot be discounted.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalJournal of Cellular Physiology
Volume178
Issue number1
DOIs
StatePublished - Jan 1999

Fingerprint

Lamin Type B
Heat-Shock Proteins
Heating
Nuclear Matrix
Cells
Hot Temperature
Nuclear Lamina
Heat-Shock Response
Proteins
Intermediate Filaments
Flow cytometry
Nuclear Proteins
Shock
Cell Survival
Flow Cytometry
Fever
Western Blotting
Recovery
Kinetics

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Lamin B is a prompt heat shock protein. / Dynlacht, Joseph; Story, Michael D.; Zhu, Wei Guo; Danner, Jeffrey.

In: Journal of Cellular Physiology, Vol. 178, No. 1, 01.1999, p. 28-34.

Research output: Contribution to journalArticle

Dynlacht, Joseph ; Story, Michael D. ; Zhu, Wei Guo ; Danner, Jeffrey. / Lamin B is a prompt heat shock protein. In: Journal of Cellular Physiology. 1999 ; Vol. 178, No. 1. pp. 28-34.
@article{88beb2385c4b42369384deadb56514c2,
title = "Lamin B is a prompt heat shock protein",
abstract = "The cellular response to hyperthermia involves the increased synthesis of heat shock proteins (HSPs) within several hours after treatment. In addition, a subset of proteins has been shown to be increased immediately after heating. These 'prompt' HSPs are predominantly found in the nuclear matrix-intermediate filament fraction and are not present or detectable in unheated cells. Since the nuclear matrix has been suggested to be a target for heat-induced cell killing, prompt HSPs may play a prominent role in the heat shock response. Using Western blotting and flow cytometry, we found that an increase in the synthesis of lamin B, one of the major proteins of the nuclear lamina, is induced during heating at 45.5°C but not during heating at 42°C. Since it is an abundant protein which is constitutively expressed in mammalian cells, lamin B appears to be a unique member of the prompt HSP family. The kinetics of induction of lamin B during 45.5°C heating did not correlate with the dose-dependent reduction in cell survival. While increased levels of lamin B during 45.5°C heating do not appear to confer a survival advantage directly, a possible role for lamin B in cellular recovery after heat shock cannot be discounted.",
author = "Joseph Dynlacht and Story, {Michael D.} and Zhu, {Wei Guo} and Jeffrey Danner",
year = "1999",
month = "1",
doi = "10.1002/(SICI)1097-4652(199901)178:1<28::AID-JCP4>3.0.CO;2-K",
language = "English",
volume = "178",
pages = "28--34",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Lamin B is a prompt heat shock protein

AU - Dynlacht, Joseph

AU - Story, Michael D.

AU - Zhu, Wei Guo

AU - Danner, Jeffrey

PY - 1999/1

Y1 - 1999/1

N2 - The cellular response to hyperthermia involves the increased synthesis of heat shock proteins (HSPs) within several hours after treatment. In addition, a subset of proteins has been shown to be increased immediately after heating. These 'prompt' HSPs are predominantly found in the nuclear matrix-intermediate filament fraction and are not present or detectable in unheated cells. Since the nuclear matrix has been suggested to be a target for heat-induced cell killing, prompt HSPs may play a prominent role in the heat shock response. Using Western blotting and flow cytometry, we found that an increase in the synthesis of lamin B, one of the major proteins of the nuclear lamina, is induced during heating at 45.5°C but not during heating at 42°C. Since it is an abundant protein which is constitutively expressed in mammalian cells, lamin B appears to be a unique member of the prompt HSP family. The kinetics of induction of lamin B during 45.5°C heating did not correlate with the dose-dependent reduction in cell survival. While increased levels of lamin B during 45.5°C heating do not appear to confer a survival advantage directly, a possible role for lamin B in cellular recovery after heat shock cannot be discounted.

AB - The cellular response to hyperthermia involves the increased synthesis of heat shock proteins (HSPs) within several hours after treatment. In addition, a subset of proteins has been shown to be increased immediately after heating. These 'prompt' HSPs are predominantly found in the nuclear matrix-intermediate filament fraction and are not present or detectable in unheated cells. Since the nuclear matrix has been suggested to be a target for heat-induced cell killing, prompt HSPs may play a prominent role in the heat shock response. Using Western blotting and flow cytometry, we found that an increase in the synthesis of lamin B, one of the major proteins of the nuclear lamina, is induced during heating at 45.5°C but not during heating at 42°C. Since it is an abundant protein which is constitutively expressed in mammalian cells, lamin B appears to be a unique member of the prompt HSP family. The kinetics of induction of lamin B during 45.5°C heating did not correlate with the dose-dependent reduction in cell survival. While increased levels of lamin B during 45.5°C heating do not appear to confer a survival advantage directly, a possible role for lamin B in cellular recovery after heat shock cannot be discounted.

UR - http://www.scopus.com/inward/record.url?scp=3743147224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3743147224&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4652(199901)178:1<28::AID-JCP4>3.0.CO;2-K

DO - 10.1002/(SICI)1097-4652(199901)178:1<28::AID-JCP4>3.0.CO;2-K

M3 - Article

C2 - 9886487

AN - SCOPUS:3743147224

VL - 178

SP - 28

EP - 34

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 1

ER -