Laminin-coated multifilament entubulation, combined with Schwann cells and glial cell line-derived neurotrophic factor, promotes unidirectional axonal regeneration in a rat model of thoracic spinal cord hemisection

Ling Xiao Deng, Nai Kui Liu, Ryan Wen, Shuang Ni Yang, Xuejun Wen, Xiao Ming Xu

Research output: Contribution to journalArticle

Abstract

Biomaterial bridging provides physical substrates to guide axonal growth across the lesion. To achieve efficient directional guidance, combinatory strategies using permissive matrix, cells and trophic factors are necessary. In the present study, we evaluated permissive effect of poly (acrylonitrile-co-vinyl chloride) guidance channels filled by different densities of laminin-precoated unidirectional polypropylene filaments combined with Schwann cells, and glial cell line-derived neurotrophic factor for axonal regeneration through a T10 hemisected spinal cord gap in adult rats. We found that channels with filaments significantly reduced the lesion cavity, astrocytic gliosis, and inflammatory responses at the graft-host boundaries. The laminin coated low density filament provided the most favorable directional guidance for axonal regeneration which was enhanced by co-grafting of Schwann cells and glial cell line-derived neurotrophic factor. These results demonstrate that the combinatorial strategy of filament-filled guiding scaffold, adhesive molecular laminin, Schwann cells, and glial cell line-derived neurotrophic factor, provides optimal topographical cues in stimulating directional axonal regeneration following spinal cord injury. This study was approved by Indiana University Institutional Animal Care and Use Committees (IACUC #:11011) on October 29, 2015.

Original languageEnglish (US)
Pages (from-to)186-191
Number of pages6
JournalNeural Regeneration Research
Volume16
Issue number1
DOIs
StatePublished - Jan 2021

Keywords

  • axonal regeneration
  • extracellular molecule
  • filament density
  • hemisection
  • laminin
  • neurotrophic factor
  • Schwann cell
  • spinal cord injury
  • thoracic
  • transplantation

ASJC Scopus subject areas

  • Developmental Neuroscience

Fingerprint Dive into the research topics of 'Laminin-coated multifilament entubulation, combined with Schwann cells and glial cell line-derived neurotrophic factor, promotes unidirectional axonal regeneration in a rat model of thoracic spinal cord hemisection'. Together they form a unique fingerprint.

  • Cite this