LAMP-2-deficient human B cells exhibit altered MHC class II presentation of exogenous antigens

Victoria L. Crotzer, Nicole Glosson, Delu Zhou, Ichizo Nishino, Janice Blum

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Major histocompatibility complex (MHC) class II molecules present antigenic peptides derived from engulfed exogenous proteins to CD4+ T cells. Exogenous antigens are processed in mature endosomes and lysosomes where acidic proteases reside and peptide-binding to class II alleles is favoured. Hence, maintenance of the microenvironment within these organelles is probably central to efficient MHC class II-mediated antigen presentation. Lysosome-associated membrane proteins such as LAMP-2 reside in mature endosomes and lysosomes, yet their role in exogenous antigen presentation pathways remains untested. In this study, human B cells lacking LAMP-2 were examined for changes in MHC class II-restricted antigen presentation. MHC class II presentation of exogenous antigen and peptides to CD4+ T cells was impaired in the LAMP-2-deficient B cells. Peptide-binding to MHC class II on LAMP-2-deficient B cells was reduced at physiological pH compared with wild-type cells. However, peptide-binding and class II-restricted antigen presentation were restored by incubation of LAMP-2-negative B cells at acidic pH, suggesting that efficient loading of exogenous epitopes by MHC class II molecules is dependent upon LAMP-2 expression in B cells. Interestingly, class II presentation of an epitope derived from an endogenous transmembrane protein was detected using LAMP-2-deficient B cells. Consequently, LAMP-2 may control the repertoire of peptides displayed by MHC class II molecules on B cells and influence the balance between endogenous and exogenous antigen presentation.

Original languageEnglish
Pages (from-to)318-330
Number of pages13
JournalImmunology
Volume131
Issue number3
DOIs
StatePublished - Nov 2010

Fingerprint

Antigen Presentation
Major Histocompatibility Complex
B-Lymphocytes
Peptides
Histocompatibility Antigens Class II
Endosomes
Lysosomes
Epitopes
Lysosome-Associated Membrane Glycoproteins
T-Lymphocytes
Organelles
Proteins
Peptide Hydrolases
Alleles
Maintenance
Antigens

Keywords

  • Exogenous antigens
  • Human B cells
  • MHC class II presentation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

LAMP-2-deficient human B cells exhibit altered MHC class II presentation of exogenous antigens. / Crotzer, Victoria L.; Glosson, Nicole; Zhou, Delu; Nishino, Ichizo; Blum, Janice.

In: Immunology, Vol. 131, No. 3, 11.2010, p. 318-330.

Research output: Contribution to journalArticle

Crotzer, Victoria L. ; Glosson, Nicole ; Zhou, Delu ; Nishino, Ichizo ; Blum, Janice. / LAMP-2-deficient human B cells exhibit altered MHC class II presentation of exogenous antigens. In: Immunology. 2010 ; Vol. 131, No. 3. pp. 318-330.
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