Abstract
Recent developments in automated optical sectioning microscope systems have enabled researchers to conduct high resolution, three-dimensional (3D) microscopy at the scale of millimeters in various types of tissues. This powerful technology allows the exploration of tissues at an unprecedented level of detail, while preserving the spatial context. By doing so, such technology will also enable researchers to explore cellular and molecular signatures within tissue and correlate with disease course. This will allow an improved understanding of pathophysiology and facilitate a precision medicine approach to assess the response to treatment. The ability to perform large-scale imaging in 3D cannot be realized without the widespread availability of accessible quantitative analysis. In this review, we will outline recent advances in large-scale 3D imaging and discuss the available methodologies to perform meaningful analysis and potential applications in translational research.
| Original language | English (US) |
|---|---|
| Journal | Translational Research |
| DOIs | |
| State | Accepted/In press - 2017 |
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ASJC Scopus subject areas
- Medicine(all)
- Public Health, Environmental and Occupational Health
- Biochemistry, medical
Cite this
Large-scale 3-dimensional quantitative imaging of tissues : State-of-the-art and translational implications. / Winfree, Seth; Ferkowicz, Michael J.; Dagher, Pierre; Kelly, Katherine; Eadon, Michael; Sutton, Timothy; Markel, Troy A.; Yoder, Mervin; Dunn, Kenneth; Ashkar (El-Achkar), Tarek.
In: Translational Research, 2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Large-scale 3-dimensional quantitative imaging of tissues
T2 - State-of-the-art and translational implications
AU - Winfree, Seth
AU - Ferkowicz, Michael J.
AU - Dagher, Pierre
AU - Kelly, Katherine
AU - Eadon, Michael
AU - Sutton, Timothy
AU - Markel, Troy A.
AU - Yoder, Mervin
AU - Dunn, Kenneth
AU - Ashkar (El-Achkar), Tarek
PY - 2017
Y1 - 2017
N2 - Recent developments in automated optical sectioning microscope systems have enabled researchers to conduct high resolution, three-dimensional (3D) microscopy at the scale of millimeters in various types of tissues. This powerful technology allows the exploration of tissues at an unprecedented level of detail, while preserving the spatial context. By doing so, such technology will also enable researchers to explore cellular and molecular signatures within tissue and correlate with disease course. This will allow an improved understanding of pathophysiology and facilitate a precision medicine approach to assess the response to treatment. The ability to perform large-scale imaging in 3D cannot be realized without the widespread availability of accessible quantitative analysis. In this review, we will outline recent advances in large-scale 3D imaging and discuss the available methodologies to perform meaningful analysis and potential applications in translational research.
AB - Recent developments in automated optical sectioning microscope systems have enabled researchers to conduct high resolution, three-dimensional (3D) microscopy at the scale of millimeters in various types of tissues. This powerful technology allows the exploration of tissues at an unprecedented level of detail, while preserving the spatial context. By doing so, such technology will also enable researchers to explore cellular and molecular signatures within tissue and correlate with disease course. This will allow an improved understanding of pathophysiology and facilitate a precision medicine approach to assess the response to treatment. The ability to perform large-scale imaging in 3D cannot be realized without the widespread availability of accessible quantitative analysis. In this review, we will outline recent advances in large-scale 3D imaging and discuss the available methodologies to perform meaningful analysis and potential applications in translational research.
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UR - http://www.scopus.com/inward/citedby.url?scp=85028296833&partnerID=8YFLogxK
U2 - 10.1016/j.trsl.2017.07.006
DO - 10.1016/j.trsl.2017.07.006
M3 - Article
C2 - 28784428
AN - SCOPUS:85028296833
JO - Translational Research
JF - Translational Research
SN - 1931-5244
ER -