Large-scale 3-dimensional quantitative imaging of tissues

State-of-the-art and translational implications

Seth Winfree, Michael J. Ferkowicz, Pierre Dagher, Katherine Kelly, Michael Eadon, Timothy Sutton, Troy A. Markel, Mervin Yoder, Kenneth Dunn, Tarek Ashkar (El-Achkar)

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

Recent developments in automated optical sectioning microscope systems have enabled researchers to conduct high resolution, three-dimensional (3D) microscopy at the scale of millimeters in various types of tissues. This powerful technology allows the exploration of tissues at an unprecedented level of detail, while preserving the spatial context. By doing so, such technology will also enable researchers to explore cellular and molecular signatures within tissue and correlate with disease course. This will allow an improved understanding of pathophysiology and facilitate a precision medicine approach to assess the response to treatment. The ability to perform large-scale imaging in 3D cannot be realized without the widespread availability of accessible quantitative analysis. In this review, we will outline recent advances in large-scale 3D imaging and discuss the available methodologies to perform meaningful analysis and potential applications in translational research.

Original languageEnglish (US)
JournalTranslational Research
DOIs
StateAccepted/In press - 2017

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Tissue
Imaging techniques
Research Personnel
Technology
Precision Medicine
Translational Medical Research
Medicine
Microscopy
Microscopic examination
Microscopes
Availability
Chemical analysis
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)
  • Public Health, Environmental and Occupational Health
  • Biochemistry, medical

Cite this

Large-scale 3-dimensional quantitative imaging of tissues : State-of-the-art and translational implications. / Winfree, Seth; Ferkowicz, Michael J.; Dagher, Pierre; Kelly, Katherine; Eadon, Michael; Sutton, Timothy; Markel, Troy A.; Yoder, Mervin; Dunn, Kenneth; Ashkar (El-Achkar), Tarek.

In: Translational Research, 2017.

Research output: Contribution to journal › Article

Winfree, S, Ferkowicz, MJ, Dagher, P, Kelly, K, Eadon, M, Sutton, T, Markel, TA, Yoder, M, Dunn, K & Ashkar (El-Achkar), T 2017, 'Large-scale 3-dimensional quantitative imaging of tissues: State-of-the-art and translational implications', Translational Research. https://doi.org/10.1016/j.trsl.2017.07.006
Winfree S, Ferkowicz MJ, Dagher P, Kelly K, Eadon M, Sutton T et al. Large-scale 3-dimensional quantitative imaging of tissues: State-of-the-art and translational implications. Translational Research. 2017. https://doi.org/10.1016/j.trsl.2017.07.006
Winfree, Seth ; Ferkowicz, Michael J. ; Dagher, Pierre ; Kelly, Katherine ; Eadon, Michael ; Sutton, Timothy ; Markel, Troy A. ; Yoder, Mervin ; Dunn, Kenneth ; Ashkar (El-Achkar), Tarek. / Large-scale 3-dimensional quantitative imaging of tissues : State-of-the-art and translational implications. In: Translational Research. 2017.
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