Large-scale mobilization and isolation of CD34+ cells from normal donors

James Croop, R. Cooper, R. Seshadri, C. Fernandez, V. Graves, S. Kreissman, F. O. Smith, Kenneth Cornetta, D. A. Williams, Rafat Abonour

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We describe collection and purification of peripheral blood CD34+ cells from volunteer, normal donors and allogenic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6 × 1010 which included 1.9 × 108 CD34+ cells corresponding to 2.7 × 106 CD34+ cells/kg. The number of CD34+ cells collected did not differ between males and females but did correlate with the donor's weight and the total number of nucleated cells collected. The Nexell Isolex 300i cell separator was used to isolate CD34+ cells from 30 of the collections. A mean of 0.36% of the total cells was recovered and included 43 ± 18% of the CD34+ cells. CD34+ cells represented 85 ± 11% of the recovered cells. The total number of CD34+ cells recovered was not influenced by the number of nucleated cells placed on the Isolex 300i. The percentage of CD34+ cells recovered was not related to the number of CD34+ cells placed on the Isolex 300i. The purity of the final product was influenced by the number of CD34+ cells but not the total number of nucleated cells. An additional 38 CD34+ cell isolations were performed on normal allogeneic stem cell donors with similar results. These observations further support the safety and feasibility of peripheral blood CD34+ cell collection and purification.

Original languageEnglish
Pages (from-to)1271-1279
Number of pages9
JournalBone Marrow Transplantation
Volume26
Issue number12
DOIs
StatePublished - 2000

Fingerprint

Cell Separation
Tissue Donors
Cell Count
Blood Cells
Stem Cells
Blood Component Removal
Volunteers
Healthy Volunteers
Safety
Weights and Measures

Keywords

  • Apheresis
  • CD34
  • Isolex
  • Mobilization
  • PBPC
  • PBSC

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

Large-scale mobilization and isolation of CD34+ cells from normal donors. / Croop, James; Cooper, R.; Seshadri, R.; Fernandez, C.; Graves, V.; Kreissman, S.; Smith, F. O.; Cornetta, Kenneth; Williams, D. A.; Abonour, Rafat.

In: Bone Marrow Transplantation, Vol. 26, No. 12, 2000, p. 1271-1279.

Research output: Contribution to journalArticle

Croop, J, Cooper, R, Seshadri, R, Fernandez, C, Graves, V, Kreissman, S, Smith, FO, Cornetta, K, Williams, DA & Abonour, R 2000, 'Large-scale mobilization and isolation of CD34+ cells from normal donors', Bone Marrow Transplantation, vol. 26, no. 12, pp. 1271-1279. https://doi.org/10.1038/sj.bmt.1702720
Croop, James ; Cooper, R. ; Seshadri, R. ; Fernandez, C. ; Graves, V. ; Kreissman, S. ; Smith, F. O. ; Cornetta, Kenneth ; Williams, D. A. ; Abonour, Rafat. / Large-scale mobilization and isolation of CD34+ cells from normal donors. In: Bone Marrow Transplantation. 2000 ; Vol. 26, No. 12. pp. 1271-1279.
@article{07f64e29f45d4de09274ca98415451d4,
title = "Large-scale mobilization and isolation of CD34+ cells from normal donors",
abstract = "We describe collection and purification of peripheral blood CD34+ cells from volunteer, normal donors and allogenic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6 × 1010 which included 1.9 × 108 CD34+ cells corresponding to 2.7 × 106 CD34+ cells/kg. The number of CD34+ cells collected did not differ between males and females but did correlate with the donor's weight and the total number of nucleated cells collected. The Nexell Isolex 300i cell separator was used to isolate CD34+ cells from 30 of the collections. A mean of 0.36{\%} of the total cells was recovered and included 43 ± 18{\%} of the CD34+ cells. CD34+ cells represented 85 ± 11{\%} of the recovered cells. The total number of CD34+ cells recovered was not influenced by the number of nucleated cells placed on the Isolex 300i. The percentage of CD34+ cells recovered was not related to the number of CD34+ cells placed on the Isolex 300i. The purity of the final product was influenced by the number of CD34+ cells but not the total number of nucleated cells. An additional 38 CD34+ cell isolations were performed on normal allogeneic stem cell donors with similar results. These observations further support the safety and feasibility of peripheral blood CD34+ cell collection and purification.",
keywords = "Apheresis, CD34, Isolex, Mobilization, PBPC, PBSC",
author = "James Croop and R. Cooper and R. Seshadri and C. Fernandez and V. Graves and S. Kreissman and Smith, {F. O.} and Kenneth Cornetta and Williams, {D. A.} and Rafat Abonour",
year = "2000",
doi = "10.1038/sj.bmt.1702720",
language = "English",
volume = "26",
pages = "1271--1279",
journal = "Bone Marrow Transplantation",
issn = "0268-3369",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - Large-scale mobilization and isolation of CD34+ cells from normal donors

AU - Croop, James

AU - Cooper, R.

AU - Seshadri, R.

AU - Fernandez, C.

AU - Graves, V.

AU - Kreissman, S.

AU - Smith, F. O.

AU - Cornetta, Kenneth

AU - Williams, D. A.

AU - Abonour, Rafat

PY - 2000

Y1 - 2000

N2 - We describe collection and purification of peripheral blood CD34+ cells from volunteer, normal donors and allogenic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6 × 1010 which included 1.9 × 108 CD34+ cells corresponding to 2.7 × 106 CD34+ cells/kg. The number of CD34+ cells collected did not differ between males and females but did correlate with the donor's weight and the total number of nucleated cells collected. The Nexell Isolex 300i cell separator was used to isolate CD34+ cells from 30 of the collections. A mean of 0.36% of the total cells was recovered and included 43 ± 18% of the CD34+ cells. CD34+ cells represented 85 ± 11% of the recovered cells. The total number of CD34+ cells recovered was not influenced by the number of nucleated cells placed on the Isolex 300i. The percentage of CD34+ cells recovered was not related to the number of CD34+ cells placed on the Isolex 300i. The purity of the final product was influenced by the number of CD34+ cells but not the total number of nucleated cells. An additional 38 CD34+ cell isolations were performed on normal allogeneic stem cell donors with similar results. These observations further support the safety and feasibility of peripheral blood CD34+ cell collection and purification.

AB - We describe collection and purification of peripheral blood CD34+ cells from volunteer, normal donors and allogenic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6 × 1010 which included 1.9 × 108 CD34+ cells corresponding to 2.7 × 106 CD34+ cells/kg. The number of CD34+ cells collected did not differ between males and females but did correlate with the donor's weight and the total number of nucleated cells collected. The Nexell Isolex 300i cell separator was used to isolate CD34+ cells from 30 of the collections. A mean of 0.36% of the total cells was recovered and included 43 ± 18% of the CD34+ cells. CD34+ cells represented 85 ± 11% of the recovered cells. The total number of CD34+ cells recovered was not influenced by the number of nucleated cells placed on the Isolex 300i. The percentage of CD34+ cells recovered was not related to the number of CD34+ cells placed on the Isolex 300i. The purity of the final product was influenced by the number of CD34+ cells but not the total number of nucleated cells. An additional 38 CD34+ cell isolations were performed on normal allogeneic stem cell donors with similar results. These observations further support the safety and feasibility of peripheral blood CD34+ cell collection and purification.

KW - Apheresis

KW - CD34

KW - Isolex

KW - Mobilization

KW - PBPC

KW - PBSC

UR - http://www.scopus.com/inward/record.url?scp=17744390859&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17744390859&partnerID=8YFLogxK

U2 - 10.1038/sj.bmt.1702720

DO - 10.1038/sj.bmt.1702720

M3 - Article

C2 - 11223966

AN - SCOPUS:17744390859

VL - 26

SP - 1271

EP - 1279

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

SN - 0268-3369

IS - 12

ER -