Large-scale mobilization and isolation of CD34+ cells from normal donors

J. M. Croop, R. Cooper, R. Seshadri, C. Fernandez, V. Graves, S. Kreissman, F. O. Smith, K. Cornetta, D. A. Williams, R. Abonour

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


We describe collection and purification of peripheral blood CD34+ cells from volunteer, normal donors and allogenic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6 × 1010 which included 1.9 × 108 CD34+ cells corresponding to 2.7 × 106 CD34+ cells/kg. The number of CD34+ cells collected did not differ between males and females but did correlate with the donor's weight and the total number of nucleated cells collected. The Nexell Isolex 300i cell separator was used to isolate CD34+ cells from 30 of the collections. A mean of 0.36% of the total cells was recovered and included 43 ± 18% of the CD34+ cells. CD34+ cells represented 85 ± 11% of the recovered cells. The total number of CD34+ cells recovered was not influenced by the number of nucleated cells placed on the Isolex 300i. The percentage of CD34+ cells recovered was not related to the number of CD34+ cells placed on the Isolex 300i. The purity of the final product was influenced by the number of CD34+ cells but not the total number of nucleated cells. An additional 38 CD34+ cell isolations were performed on normal allogeneic stem cell donors with similar results. These observations further support the safety and feasibility of peripheral blood CD34+ cell collection and purification.

Original languageEnglish (US)
Pages (from-to)1271-1279
Number of pages9
JournalBone marrow transplantation
Issue number12
StatePublished - 2000


  • Apheresis
  • CD34
  • Isolex
  • Mobilization
  • PBPC
  • PBSC

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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