Laser-assisted microdissection in translational research: Theory, technical considerations, and future applications

Liang Cheng, Shaobo Zhang, Gregory T. MacLennan, Sean R. Williamson, Darrell Davidson, Mingsheng Wang, Timothy D. Jones, Antonio Lopez-Beltran, Rodolfo Montironi

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Molecular profiling already exerts a profound influence on biomedical research and disease management. Microdissection technologies contribute to the molecular profiling of diseases, enabling investigators to probe genetic characteristics and dissect functional physiology within specific cell populations. Laser-capture microdissection (LCM), in particular, permits collation of genetic, epigenetic, and gene expression differences between normal, premalignant, and malignant cell populations. Its selectivity for specific cell populations promises to greatly improve the diagnosis and management of many human diseases. LCM has been extensively used in cancer research, contributing to the understanding of tumor biology by mutation detection, clonality analysis, epigenetic alteration assessment, gene expression profiling, proteomics, and metabolomics. In this review, we focus on LCM applications for DNA, RNA, and protein analysis in specific cell types and on commercially available LCM platforms. These analyses could clinically be used as aids to cancer diagnosis, clinical management, genomic profile studies, and targeted therapy. In this review, we also discuss the technical details of tissue preparation, analytical yields, tissue selection, and selected applications using LCM.

Original languageEnglish
Pages (from-to)31-47
Number of pages17
JournalApplied Immunohistochemistry and Molecular Morphology
Volume21
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Laser Capture Microdissection
Microdissection
Translational Medical Research
Lasers
Epigenomics
Population
Neoplasms
Metabolomics
Gene Expression Profiling
Disease Management
Proteomics
Biomedical Research
Research Personnel
RNA
Technology
Gene Expression
Mutation
DNA
Research
Proteins

Keywords

  • cancer heterogeneity
  • carcinogenesis
  • gene expression profiling
  • genome characterization/alteration
  • laser-capture microdissection
  • personalized medicine
  • proteomics
  • targeted therapy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology
  • Histology

Cite this

Laser-assisted microdissection in translational research : Theory, technical considerations, and future applications. / Cheng, Liang; Zhang, Shaobo; MacLennan, Gregory T.; Williamson, Sean R.; Davidson, Darrell; Wang, Mingsheng; Jones, Timothy D.; Lopez-Beltran, Antonio; Montironi, Rodolfo.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 21, No. 1, 01.2013, p. 31-47.

Research output: Contribution to journalArticle

Cheng, Liang ; Zhang, Shaobo ; MacLennan, Gregory T. ; Williamson, Sean R. ; Davidson, Darrell ; Wang, Mingsheng ; Jones, Timothy D. ; Lopez-Beltran, Antonio ; Montironi, Rodolfo. / Laser-assisted microdissection in translational research : Theory, technical considerations, and future applications. In: Applied Immunohistochemistry and Molecular Morphology. 2013 ; Vol. 21, No. 1. pp. 31-47.
@article{24656d22ca104cb6a4a238f5e0a04dab,
title = "Laser-assisted microdissection in translational research: Theory, technical considerations, and future applications",
abstract = "Molecular profiling already exerts a profound influence on biomedical research and disease management. Microdissection technologies contribute to the molecular profiling of diseases, enabling investigators to probe genetic characteristics and dissect functional physiology within specific cell populations. Laser-capture microdissection (LCM), in particular, permits collation of genetic, epigenetic, and gene expression differences between normal, premalignant, and malignant cell populations. Its selectivity for specific cell populations promises to greatly improve the diagnosis and management of many human diseases. LCM has been extensively used in cancer research, contributing to the understanding of tumor biology by mutation detection, clonality analysis, epigenetic alteration assessment, gene expression profiling, proteomics, and metabolomics. In this review, we focus on LCM applications for DNA, RNA, and protein analysis in specific cell types and on commercially available LCM platforms. These analyses could clinically be used as aids to cancer diagnosis, clinical management, genomic profile studies, and targeted therapy. In this review, we also discuss the technical details of tissue preparation, analytical yields, tissue selection, and selected applications using LCM.",
keywords = "cancer heterogeneity, carcinogenesis, gene expression profiling, genome characterization/alteration, laser-capture microdissection, personalized medicine, proteomics, targeted therapy",
author = "Liang Cheng and Shaobo Zhang and MacLennan, {Gregory T.} and Williamson, {Sean R.} and Darrell Davidson and Mingsheng Wang and Jones, {Timothy D.} and Antonio Lopez-Beltran and Rodolfo Montironi",
year = "2013",
month = "1",
doi = "10.1097/PAI.0b013e31824d0519",
language = "English",
volume = "21",
pages = "31--47",
journal = "Applied Immunohistochemistry and Molecular Morphology",
issn = "1541-2016",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Laser-assisted microdissection in translational research

T2 - Theory, technical considerations, and future applications

AU - Cheng, Liang

AU - Zhang, Shaobo

AU - MacLennan, Gregory T.

AU - Williamson, Sean R.

AU - Davidson, Darrell

AU - Wang, Mingsheng

AU - Jones, Timothy D.

AU - Lopez-Beltran, Antonio

AU - Montironi, Rodolfo

PY - 2013/1

Y1 - 2013/1

N2 - Molecular profiling already exerts a profound influence on biomedical research and disease management. Microdissection technologies contribute to the molecular profiling of diseases, enabling investigators to probe genetic characteristics and dissect functional physiology within specific cell populations. Laser-capture microdissection (LCM), in particular, permits collation of genetic, epigenetic, and gene expression differences between normal, premalignant, and malignant cell populations. Its selectivity for specific cell populations promises to greatly improve the diagnosis and management of many human diseases. LCM has been extensively used in cancer research, contributing to the understanding of tumor biology by mutation detection, clonality analysis, epigenetic alteration assessment, gene expression profiling, proteomics, and metabolomics. In this review, we focus on LCM applications for DNA, RNA, and protein analysis in specific cell types and on commercially available LCM platforms. These analyses could clinically be used as aids to cancer diagnosis, clinical management, genomic profile studies, and targeted therapy. In this review, we also discuss the technical details of tissue preparation, analytical yields, tissue selection, and selected applications using LCM.

AB - Molecular profiling already exerts a profound influence on biomedical research and disease management. Microdissection technologies contribute to the molecular profiling of diseases, enabling investigators to probe genetic characteristics and dissect functional physiology within specific cell populations. Laser-capture microdissection (LCM), in particular, permits collation of genetic, epigenetic, and gene expression differences between normal, premalignant, and malignant cell populations. Its selectivity for specific cell populations promises to greatly improve the diagnosis and management of many human diseases. LCM has been extensively used in cancer research, contributing to the understanding of tumor biology by mutation detection, clonality analysis, epigenetic alteration assessment, gene expression profiling, proteomics, and metabolomics. In this review, we focus on LCM applications for DNA, RNA, and protein analysis in specific cell types and on commercially available LCM platforms. These analyses could clinically be used as aids to cancer diagnosis, clinical management, genomic profile studies, and targeted therapy. In this review, we also discuss the technical details of tissue preparation, analytical yields, tissue selection, and selected applications using LCM.

KW - cancer heterogeneity

KW - carcinogenesis

KW - gene expression profiling

KW - genome characterization/alteration

KW - laser-capture microdissection

KW - personalized medicine

KW - proteomics

KW - targeted therapy

UR - http://www.scopus.com/inward/record.url?scp=84872005086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872005086&partnerID=8YFLogxK

U2 - 10.1097/PAI.0b013e31824d0519

DO - 10.1097/PAI.0b013e31824d0519

M3 - Article

C2 - 22495368

AN - SCOPUS:84872005086

VL - 21

SP - 31

EP - 47

JO - Applied Immunohistochemistry and Molecular Morphology

JF - Applied Immunohistochemistry and Molecular Morphology

SN - 1541-2016

IS - 1

ER -