Laser capture microdissection analysis reveals frequent allelic losses in papillary urothelial neoplasm of low malignant potential of the urinary bladder

Liang Cheng, Gregory T. MacLennan, Shaobo Zhang, Mingsheng Wang, Chong Xian Pan, Michael Koch

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Abstract

BACKGROUND. In the 1999 World Health Organization classification system, papillary tumors of the urinary bladder were classified as papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and as Grade 1, Grade 2, and Grade 3 urothelial carcinoma. The biologic potential of PUNLMP of the urinary bladder is controversial. To the authors' knowledge, information regarding the genetic changes of PUNLMP tumors of the bladder is limited. METHODS. The authors examined loss of heterogygosity (LOH) at 5 polymorphic microsatellite markers on chromosome 9q32-33 (D9S177), 9p22 (IFNA), 17p13.1 (TP53), 12q14-24 (D12S1051), and 3p25-26 (D3S3050) from 26 patients who were diagnosed with PUNLMP tumors of the urinary bladder. Tumors were microdissected from sections prepared from formalin-fixed, paraffin-processed tissue specimens using laser capture microdissection. RESULTS. LOH was found in 21 of 26 (81%) patients with PUNLMP. The rate of LOH was 41% with D9S177, 32% with IFNA, 29% with TP53, 26% with D12S1051, and 44% with D3S3050. Allelic loss of multiple chromosome loci was often present in patients with PUNLMP tumors. CONCLUSIONS. Genetic changes that commonly occur in advanced bladder carcinoma (≥ pT2) are frequently found in PUNLMP of the urinary bladder.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalCancer
Volume101
Issue number1
DOIs
StatePublished - Jul 1 2004

Fingerprint

Laser Capture Microdissection
Loss of Heterozygosity
Urinary Bladder
Neoplasms
Urinary Bladder Neoplasms
Chromosomes
Carcinoma
Papilloma
Paraffin
Microsatellite Repeats
Formaldehyde

Keywords

  • Grading
  • Laser capture microdissection
  • Loss of heterozygosity
  • Neoplasms
  • Papillary urothelial neoplasm of low malignant potential
  • Urinary bladder
  • Urothelial (transitional cell) carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

@article{dcbd703185d341759ba96b904ad0800b,
title = "Laser capture microdissection analysis reveals frequent allelic losses in papillary urothelial neoplasm of low malignant potential of the urinary bladder",
abstract = "BACKGROUND. In the 1999 World Health Organization classification system, papillary tumors of the urinary bladder were classified as papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and as Grade 1, Grade 2, and Grade 3 urothelial carcinoma. The biologic potential of PUNLMP of the urinary bladder is controversial. To the authors' knowledge, information regarding the genetic changes of PUNLMP tumors of the bladder is limited. METHODS. The authors examined loss of heterogygosity (LOH) at 5 polymorphic microsatellite markers on chromosome 9q32-33 (D9S177), 9p22 (IFNA), 17p13.1 (TP53), 12q14-24 (D12S1051), and 3p25-26 (D3S3050) from 26 patients who were diagnosed with PUNLMP tumors of the urinary bladder. Tumors were microdissected from sections prepared from formalin-fixed, paraffin-processed tissue specimens using laser capture microdissection. RESULTS. LOH was found in 21 of 26 (81{\%}) patients with PUNLMP. The rate of LOH was 41{\%} with D9S177, 32{\%} with IFNA, 29{\%} with TP53, 26{\%} with D12S1051, and 44{\%} with D3S3050. Allelic loss of multiple chromosome loci was often present in patients with PUNLMP tumors. CONCLUSIONS. Genetic changes that commonly occur in advanced bladder carcinoma (≥ pT2) are frequently found in PUNLMP of the urinary bladder.",
keywords = "Grading, Laser capture microdissection, Loss of heterozygosity, Neoplasms, Papillary urothelial neoplasm of low malignant potential, Urinary bladder, Urothelial (transitional cell) carcinoma",
author = "Liang Cheng and MacLennan, {Gregory T.} and Shaobo Zhang and Mingsheng Wang and Pan, {Chong Xian} and Michael Koch",
year = "2004",
month = "7",
day = "1",
doi = "10.1002/cncr.20343",
language = "English",
volume = "101",
pages = "183--188",
journal = "Cancer",
issn = "0008-543X",
publisher = "John Wiley and Sons Inc.",
number = "1",

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TY - JOUR

T1 - Laser capture microdissection analysis reveals frequent allelic losses in papillary urothelial neoplasm of low malignant potential of the urinary bladder

AU - Cheng, Liang

AU - MacLennan, Gregory T.

AU - Zhang, Shaobo

AU - Wang, Mingsheng

AU - Pan, Chong Xian

AU - Koch, Michael

PY - 2004/7/1

Y1 - 2004/7/1

N2 - BACKGROUND. In the 1999 World Health Organization classification system, papillary tumors of the urinary bladder were classified as papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and as Grade 1, Grade 2, and Grade 3 urothelial carcinoma. The biologic potential of PUNLMP of the urinary bladder is controversial. To the authors' knowledge, information regarding the genetic changes of PUNLMP tumors of the bladder is limited. METHODS. The authors examined loss of heterogygosity (LOH) at 5 polymorphic microsatellite markers on chromosome 9q32-33 (D9S177), 9p22 (IFNA), 17p13.1 (TP53), 12q14-24 (D12S1051), and 3p25-26 (D3S3050) from 26 patients who were diagnosed with PUNLMP tumors of the urinary bladder. Tumors were microdissected from sections prepared from formalin-fixed, paraffin-processed tissue specimens using laser capture microdissection. RESULTS. LOH was found in 21 of 26 (81%) patients with PUNLMP. The rate of LOH was 41% with D9S177, 32% with IFNA, 29% with TP53, 26% with D12S1051, and 44% with D3S3050. Allelic loss of multiple chromosome loci was often present in patients with PUNLMP tumors. CONCLUSIONS. Genetic changes that commonly occur in advanced bladder carcinoma (≥ pT2) are frequently found in PUNLMP of the urinary bladder.

AB - BACKGROUND. In the 1999 World Health Organization classification system, papillary tumors of the urinary bladder were classified as papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and as Grade 1, Grade 2, and Grade 3 urothelial carcinoma. The biologic potential of PUNLMP of the urinary bladder is controversial. To the authors' knowledge, information regarding the genetic changes of PUNLMP tumors of the bladder is limited. METHODS. The authors examined loss of heterogygosity (LOH) at 5 polymorphic microsatellite markers on chromosome 9q32-33 (D9S177), 9p22 (IFNA), 17p13.1 (TP53), 12q14-24 (D12S1051), and 3p25-26 (D3S3050) from 26 patients who were diagnosed with PUNLMP tumors of the urinary bladder. Tumors were microdissected from sections prepared from formalin-fixed, paraffin-processed tissue specimens using laser capture microdissection. RESULTS. LOH was found in 21 of 26 (81%) patients with PUNLMP. The rate of LOH was 41% with D9S177, 32% with IFNA, 29% with TP53, 26% with D12S1051, and 44% with D3S3050. Allelic loss of multiple chromosome loci was often present in patients with PUNLMP tumors. CONCLUSIONS. Genetic changes that commonly occur in advanced bladder carcinoma (≥ pT2) are frequently found in PUNLMP of the urinary bladder.

KW - Grading

KW - Laser capture microdissection

KW - Loss of heterozygosity

KW - Neoplasms

KW - Papillary urothelial neoplasm of low malignant potential

KW - Urinary bladder

KW - Urothelial (transitional cell) carcinoma

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U2 - 10.1002/cncr.20343

DO - 10.1002/cncr.20343

M3 - Article

VL - 101

SP - 183

EP - 188

JO - Cancer

JF - Cancer

SN - 0008-543X

IS - 1

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